Traditional Chinese medicine extract for treating hepatitis B, and preparation method and application thereof
A hepatitis B, extract technology, applied in the field of medicine, to achieve a good dose-dependent relationship, the effect of reducing hepatic steatosis
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Embodiment 1
[0077] Take 50kg of neem bark, heat and reflux with 400L deionized water to extract 3 times, the extraction time is 2, 2, and 1 hour respectively, the water extracts are combined, filtered, concentrated under reduced pressure at 60°C to a relative density of 1.05, and added to the concentrated solution Ethanol, until the ethanol concentration is 80%, precipitate overnight, filter, the filtrates are combined, the solvent is recovered under reduced pressure to a concentration of about 2.0g crude drug / ml, filter, the macroporous adsorption resin column HZ816 on the filtrate, the resin consumption is 50kg, control The flow rate is 0.2~0.5 times the column volume / h, successively eluted with 200L deionized water, 150L 10% ethanol to remove impurities, and then eluted with 150L 70% ethanol, collected the alcohol eluate, concentrated under reduced pressure, dried in vacuum, and pulverized , to obtain neem extract 240g, the total phenolic compound content is 52%, and the neem phenolic c...
Embodiment 2
[0079] Take 15 kg of Toosendan, heat and reflux with 90L deionized water to extract 3 times, the extraction time is 2, 2, and 1.5 hours respectively, the water extracts are combined, filtered, and concentrated under reduced pressure at 60 ° C to a relative density of 1.05; Add ethanol until the ethanol concentration is 90%, precipitate overnight, filter, combine the filtrates, recover the solvent under reduced pressure to a concentration of about 2.0g crude drug / ml, filter, put the filtrate on the resin column HP10, the resin consumption is 15kg, and the control flow rate is 0.2~0.5 times of column volume / h, eluted with 30L deionized water, 30L 10% ethanol to remove impurities, and then eluted with 40L 80% ethanol, collected the alcohol eluate, concentrated under reduced pressure, dried in vacuum, and pulverized to obtain Neem extract 80g, the total phenolic compound content is 53%, and the neem phenolic compound content is 21.7%.
Embodiment 3
[0081] Take 150kg of Neem leaves, heat and reflux with 900L 80% ethanol to extract twice, the extraction time is 3 and 2 hours respectively, the ethanol extracts are combined, filtered, concentrated under reduced pressure at 60°C, and the solvent is recovered to a concentration of about 2.0g crude drug / ml, filtered, and the filtrate was put on a macroporous adsorption resin column HZ801, the amount of resin was 120kg, the flow rate was controlled to be 0.2~0.5 times column volume / h, eluted with 300L deionized water and 250L 10% ethanol successively to remove impurities, and then used Eluted with 250L 60% ethanol, collected the ethanol eluent, concentrated under reduced pressure, dried in vacuum, and pulverized to obtain 906g of neem extract, with a total phenolic compound content of 57% and neem phenolic compound content of 24.5%.
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