Biological detection method for small ubiquitin-like modification
A small ubiquitin-like, biological detection technology, applied in the field of small ubiquitin-like modified biological detection, can solve the problems of low sensitivity, large influence of antibody titer and specificity, and unfavorable wide application.
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[0019] The biological detection method of small ubiquitin-like modification will be further described in detail mainly in conjunction with the accompanying drawings and specific examples below.
[0020] Such as figure 1 As shown, the biological detection method of small ubiquitin-like modification in one embodiment includes the following steps:
[0021] Step S110, constructing the fusion gene of the small ubiquitin-like modifier protein gene and the green fluorescent protein gene, which specifically includes the following steps:
[0022] First use PCR technology to amplify the small ubiquitin-like modifier protein gene; then, under the action of restriction endonucleases, through in vitro ligation reaction, the amplified small ubiquitin-like modifier protein gene is integrated into the vector containing the green fluorescent protein gene In this method, the small ubiquitin-like modifier protein gene is fused with the green fluorescent protein gene to obtain a fusion gene cont...
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