Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for preparing stem cells from human amniotic membrane

A technology for amniotic membrane epithelial cells and stem cells is applied in the field of separation of epithelial cells and mesenchymal stem cells in amniotic membrane, which can solve the problems of poor cell activity, affecting cell separation and culture, and high pollution rate of amniotic membrane collection.

Active Publication Date: 2017-01-18
山西省干细胞基因工程有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention discloses a method for preparing stem cells from human amniotic membrane, which is used to solve the problems of high amniotic membrane collection pollution rate and poor cell activity after collection in the prior art, which directly affects subsequent cell separation and culture

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for preparing stem cells from human amniotic membrane
  • A method for preparing stem cells from human amniotic membrane
  • A method for preparing stem cells from human amniotic membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0121] The present invention will be further described below in conjunction with accompanying drawing and embodiment:

[0122]A method for preparing stem cells from human amniotic membrane, which includes collecting, separating, culturing, freezing, and detecting; wherein, during the separation process, the amniotic membrane epithelial cells are digested with enzymes, and the amniotic membrane mesenchymal stem cells are then sliced ​​by tissue method, the two are completed step by step.

[0123] The collection of the present invention specifically includes: putting the collected placenta into a sterile bag containing D-Hanks liquid placenta protection solution of 0.2 g / L streptomycin and 0.12 g / L penicillin, sealing the sterile bag and putting Put the collection box into the collection box, put the collection box into the constant temperature collection box, and send it to the stem cells within 24 hours with the constant temperature collection box.

[0124] The placenta colle...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing stem cells from a human amniotic membrane, specifically relates to a method for separating epithelial cells and mesenchymal stem cells from the amniotic membrane, and relates to the technical field of cell separation. The method comprises the following steps: collecting a placenta by self-made sterile, efficient and stable placenta protecting liquid; and separating amniotic membrane epithelial cells and amniotic membrane mesenchymal stem cells by combining a digestion process and a climbing process to respectively obtain high-purity amniotic membrane epithelial cells and high-purity amniotic membrane mesenchymal stem cells. The method disclosed by the invention solves a problem that subsequent cell separation and culture is directly affected by high human amniotic membrane collecting pollution rate and poor activity of the collected cells in the prior art. According to the method disclosed by the invention, the method for separating the stem cells from the amniotic membrane is improved and created to form a standard separating scheme, so that the stem cell resource can be utilized more stably and sufficiently, pollution is avoided, purity is high and the number of the cells is large; while the cell activity is kept, original characteristics of the stem cells are ensured.

Description

technical field [0001] The invention relates to a cell separation technology, in particular to a method for separating epithelial cells and mesenchymal stem cells from amnion. Background technique [0002] Amnion is the product of embryonic development. Amnion cells have stem cell characteristics and do not express human leukocyte antigens HLA-I and HLA- , There is no allogeneic immune rejection, so it is suitable for cell therapy. The stem cells in the amniotic membrane mainly include epithelial cells and mesenchymal stem cells. [0003] Amnion epithelial cells have the characteristics of subtotipotent stem cells, have strong differentiation ability, can differentiate into three germ layers, and have no tumorigenicity and ethical problems. They are ideal seed cells for tissue engineering and cell therapy. Amniotic mesenchymal stem cells have a certain multi-directional differentiation potential, but compared with amniotic epithelial cells, their main feature is that they ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0735C12N5/0775C12Q1/04C12Q1/68G01N15/14
Inventor 徐志国贡波薛飞虞焰志刘拥军路建伟朱德琳章靖王学军
Owner 山西省干细胞基因工程有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com