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A kind of extraction technology of bletilla striata cyclopine-type triterpene and its application

A technology of jackfruit alkane and extraction process, which is applied in the directions of steroids, antitumor drugs, organic chemistry, etc., can solve the problems of separation, purification and functional research of triterpenoids that have not been seen, and achieves solvent safety, simple process, and avoidance of pollution. Effect

Active Publication Date: 2015-12-30
HUIZHOU UNIV
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  • Application Information

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Problems solved by technology

The paper only has one short page, reporting that there are 2 kinds of steroids (steroids) compounds and 5 kinds of triterpenoids (also known as: cycloartene, cycloaltinane, etc.) in Bletilla striata. ), but there is no detailed report on the separation, purification and functional research of these five triterpenoids

Method used

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  • A kind of extraction technology of bletilla striata cyclopine-type triterpene and its application
  • A kind of extraction technology of bletilla striata cyclopine-type triterpene and its application
  • A kind of extraction technology of bletilla striata cyclopine-type triterpene and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1: Extraction of Bletilla striata cyclopine type triterpenes

[0037] (1) Extraction and concentration: buy 30kg of bletilla striata roots from the market of traditional Chinese medicinal materials, air-dry them and grind them with a traditional Chinese medicine grinder to obtain about 27kg of bletilla striata powder, divide them into 1000mL beakers, add 75% ethanol to moisten and soak them, and use an ultrasonic cleaner Ultrasonic treatment was performed for 30 minutes, and then placed in an 80°C water bath to heat and keep warm for 2 hours to accelerate the dissolution of the effective active ingredients of Bletilla striata powder. The volume of 75% ethanol leaching and percolation treatment, after standing for one week, the percolation solution was collected. Concentrate the percolation solution with a rotary evaporator under normal pressure to obtain the extract. Percolation treatment was performed four times, concentrated by rotary evaporation for several ...

Embodiment 2

[0046] Implementation 2: Extraction of Bletilla striata cyclopine-type triterpenes

Embodiment 2

[0047] The raw materials and operating steps of Example 2 are basically the same as those in Example 1, except that some parameters and reagents of the experimental process are properly adjusted. For example, the alcohol used in the extraction and concentration process is 95% ethanol, and the temperature of the water bath heating is 90 ° C; During the extraction and concentration process, the volume ratio of petroleum ether to the extract suspension solution was 1:3, and the number of extractions was 5 times.

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Abstract

The invention provides an extraction process and application of bletilla striata cycloartane triterpenoid. According to the invention, bletilla striata is used as a raw material, and the steps of leaching with alcohol, ultrasonic treatment, percoltation, evaporation, concentration, extraction with petroleum ether, silica gel column chromatography separation, thin-layer chromatography detection, crystallization, re-crystallization and the like are mainly performed to obtain a pure white product of bletilla striata cycloartane triterpenoid, and the purity of the product reaches as high as 99%. The extraction process is simple, the extraction rate is high, and the bletilla striata cycloartane triterpenoid can be produced in large scale. The invention discloses an extraction process of the bletilla striata cycloartane triterpenoid for the first time, which facilitates the further study on the anti-tumor mechanism of bletilla striata serving as a vascular occlusive agent. The cycloartane triterpenoid is very likely to be a potential small anti-cancer molecule or angiogenesis inhibitor.

Description

technical field [0001] The invention relates to a component extraction process using bletilla striata as a raw material, in particular to an extraction process and application of bletilla striata cyclopine-type triterpenes, belonging to the technical field of traditional Chinese medicine. Background technique [0002] Bletilla striata (scientific name: Bletilla striata) is a perennial herbaceous bulbous plant with astringent and hemostatic effects. In China, for many years, the main use of Bletilla striata is as a vascular embolism agent in tumor interventional therapy. It has obvious curative effect on the treatment of primary liver cancer and transplanted liver cancer and has been widely used in clinical practice. In order to elucidate the molecular mechanism of Bletilla striata as a vascular embolic agent to inhibit tumor at the molecular level, the research on the biological function of Bletilla striata must finally go deep into the study of the chemical composition of B...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J53/00A61P35/00
Inventor 刘明志
Owner HUIZHOU UNIV
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