Polypeptide capable of inhibiting human immunodeficiency virus infection activity, and related bacteriophage

A technology of AIDS virus and phage, applied in the direction of virus/bacteriophage, antiviral agent, peptide, etc., can solve difficult problems such as success

Inactive Publication Date: 2014-04-09
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the field of HIV prevention, effective preventiv

Method used

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  • Polypeptide capable of inhibiting human immunodeficiency virus infection activity, and related bacteriophage
  • Polypeptide capable of inhibiting human immunodeficiency virus infection activity, and related bacteriophage
  • Polypeptide capable of inhibiting human immunodeficiency virus infection activity, and related bacteriophage

Examples

Experimental program
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Effect test

Embodiment 1

[0021] 1. Screening and identification of T7P polypeptide sequence and T7M phage

[0022] Using phage display technology, phage screening was carried out using the polypeptide (T20) of the CHR region and MPER region on HIV-1gp41 as bait; the phage obtained from the screening and binding to the bait protein were amplified and sequenced for identification. The specific method is as follows:

[0023] (1) Add the phage eluate to 40ml ER2738 culture, shake vigorously at 37°C for 4h;

[0024] (2) Transfer the culture to a centrifuge tube, centrifuge at 10,000 rpm for 10 min at 4°C, transfer the supernatant to another centrifuge tube, and centrifuge again;

[0025] (3) Transfer the upper 80% of the supernatant to a fresh tube, add 1 / 6 volume of PEG 8000 / 2.5M NaCl, and precipitate overnight at 4°C;

[0026] (4) Centrifuge at 10,000 rpm for 15 minutes at 4°C, and discard the supernatant;

[0027] (5) Resuspend the precipitate in 1ml Tris buffer solution (pH=7.4), centrifuge briefly, ...

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Abstract

The invention belongs to the field of biomedicine and antiviral biological agents, relates to a polypeptide capable of inhibiting human immunodeficiency virus (HIV-1) infection activity, and a related bacteriophage, and especially relates to a polypeptide with in vitro inhibitory effects on human immunodeficiency virus (HIV-1) infection activity, and a bacteriophage containing sequence of the polypeptide. The polypeptide and the bacteriophage possesses in vitro inhibitory effects on human immunodeficiency virus (HIV-1) infection activity, and low toxicity on cells; the polypeptide, the bacteriophage and derivatives thereof can be used for exclusive use, or combined with other ingredients so as to develop and prepare anti-HIV drugs and biological agents used for preventing HIV-1 sexual transmission, and provide novel thoughts and novel technology for blocking male to male rectum sexual transmission of HIV.

Description

technical field [0001] The invention belongs to the field of biomedicine and antiviral biological preparations, and relates to polypeptides and related bacteriophages for inhibiting HIV-1 infection in vitro, in particular to a polypeptide with activity of inhibiting HIV-1 infection in vitro and containing Phage of the polypeptide sequence. The polypeptide and its bacteriophage can be used to prepare anti-HIV-1 medicines and preparations for preventing sexual transmission of HIV-1. Background technique [0002] The global spread of human immunodeficiency virus (Human immunodeficiency virus, HIV) and AIDS caused by it has become a serious public health problem. At present, the effective prevention and thorough treatment of this disease are still a worldwide problem. According to the 2011 China AIDS Epidemic Assessment Report jointly made by the Ministry of Health, UNAIDS, and the World Health Organization, by the end of 2011, it was estimated that there were about 780,000 liv...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12N7/01A61K38/08A61P31/18
Inventor 姜世勃陆路李雯徐巍
Owner FUDAN UNIV
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