Polypeptide capable of inhibiting human immunodeficiency virus infection activity, and related bacteriophage
A technology of AIDS virus and phage, applied in the direction of virus/bacteriophage, antiviral agent, peptide, etc., can solve difficult problems such as success
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[0021] 1. Screening and identification of T7P polypeptide sequence and T7M phage
[0022] Using phage display technology, phage screening was carried out using the polypeptide (T20) of the CHR region and MPER region on HIV-1gp41 as bait; the phage obtained from the screening and binding to the bait protein were amplified and sequenced for identification. The specific method is as follows:
[0023] (1) Add the phage eluate to 40ml ER2738 culture, shake vigorously at 37°C for 4h;
[0024] (2) Transfer the culture to a centrifuge tube, centrifuge at 10,000 rpm for 10 min at 4°C, transfer the supernatant to another centrifuge tube, and centrifuge again;
[0025] (3) Transfer the upper 80% of the supernatant to a fresh tube, add 1 / 6 volume of PEG 8000 / 2.5M NaCl, and precipitate overnight at 4°C;
[0026] (4) Centrifuge at 10,000 rpm for 15 minutes at 4°C, and discard the supernatant;
[0027] (5) Resuspend the precipitate in 1ml Tris buffer solution (pH=7.4), centrifuge briefly, ...
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