Kit for early diagnosis of papillary thyroid carcinoma

A technique for the early diagnosis of papillary carcinoma, applied in the field of molecular biology, can solve problems such as suspicious and uncertain diagnosis results of thyroid cancer

Inactive Publication Date: 2014-04-09
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The diagnosis of thyroid cancer mainly relies on thyroid ultrasonography and needle biopsy pathology at present. Although the diagnosis of thyroid cancer by biopsy pathology has been widely used in clinical practice, there are still 30% suspicious or uncertain diagnostic results of thyroid cancer, and biopsy is a Invasive examination brings great pain to patients

Method used

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  • Kit for early diagnosis of papillary thyroid carcinoma
  • Kit for early diagnosis of papillary thyroid carcinoma
  • Kit for early diagnosis of papillary thyroid carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] A kit for early diagnosis of thyroid papillary carcinoma, comprising: primers such as sequence listing SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, Shown in SEQ IDNO.7 and SEQ IDNO.8, the composition of other liquids in the kit is as follows:

[0062] Premix Ex Taq TM II (Tli RNaseH Plus) (2×Conc.) 1ml / tube 15 tubes / box colorless transparent liquid;

[0063] ROX Reference Dye (50×Conc.) 200μl / tube 3 tubes / box Orange-yellow transparent liquid;

[0064] ROX Reference Dye II (50×Conc.) 200μl / tube 3 tubes / box orange-yellow transparent liquid;

[0065] TP53INP1 upstream and downstream primer mixture (10μM) 400μl / tube 1 tube / box Colorless transparent liquid;

[0066] TP53INP2 upstream and downstream primer mixture (10μM) 400μl / tube 1 tube / box Colorless transparent liquid;

[0067] AXIN2 upstream and downstream primer mixture (10μM) 400μl / tube 1 tube / box Colorless transparent liquid;

[0068] GAPDH upstream and downstream primer mixture ...

Embodiment 2

[0075] The detection method and specific operation steps of the kit for early diagnosis of papillary thyroid carcinoma:

[0076] Primers were designed according to the conserved sequences TP53INP1, TP53INP2, AXIN2 and GAPDH genes. The primer sequences are as follows:

[0077] TP53INP1

[0078] Sense: 5'-CTTAACAATGTTCCAGAGGCTGAAT-3'

[0079] Antisense: 5'-AGGGTGCTCAGT AGGTGACTCTT-3'

[0080] TP53INP2

[0081] Sense:5'-:5'-GGTCCGTGTTGCACCTAA-3'

[0082] Antisense: 5'-TTGGATCCCCACCATGGAGGGCGC-3'

[0083] AXIN2

[0084] Sense: 5’-5’-CACGGAAACTGTTGACAGTGGATAC-3’

[0085] Antisense: 5'-GGTGGCTGGTGC AAAGACATAG-3'

[0086] GAPDH

[0087] Sense: 5'-GCACCGTC AAGGCTGAGAAC-3'

[0088] Antisense: 5'-TGGTGAAGACGCCAGTGGA-3'

[0089] Collection of peripheral blood samples to be detected: 82 cases of peripheral blood samples to be detected were collected using EDTA anticoagulant blood collection tubes. The specific steps of blood sample collection in this experiment include:

[0090...

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Abstract

The invention discloses a kit for early diagnosis of papillary thyroid carcinoma. A primer sequence is as follows: TP53INP1 Sense:5'-CTTAACAATGTTCCAGAGGCTGAAT-3'Antisense:5'-AGGGTGCTCAGT AGGTGACTCTT-3'; TP53INP2Sense:5'-:5'-GGTCCGTGTTGCACCTAA-3'; Antisense:5'-TTGGATCCCACCATGGAGGGCGC-3'AXIN2Sense:5'-5'-CACGGAAACTGTTGACAGTGGATAC-3'; Antisense:5'-GGTGGCTGGTGC AAAGACATAG-3'' GAPDH Sense:5'-GCACCGTC AAGGCTGAGAAC-3'; and Antisense:5'-TGGTGAAGACGCCCAGTGGA-3'. The kit disclosed by the invention has high sensitivity and high specificity, and can be used for a screening and early diagnosis technology of the papillary thyroid carcinoma.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to a kit for early diagnosis of thyroid papillary carcinoma. Background technique [0002] At present, human thyroid cancer is one of the more common endocrine malignancies, and its incidence has been increasing year by year in recent years. The average age of onset is 50 years old, and most of them are women. Among them, papillary thyroid carcinoma (PTC) has the highest incidence rate, accounting for about 80% of all incidence types. The diagnosis of thyroid cancer mainly relies on thyroid ultrasonography and needle biopsy pathology at present. Although the diagnosis of thyroid cancer by biopsy pathology has been widely used in clinical practice, there are still 30% suspicious or uncertain diagnostic results of thyroid cancer, and biopsy is a Invasive examination brings great pain to patients. Thyroid cancer is generally treated with surgery. The five-year survival rate of patien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/158
Inventor 刘晓冬李文兴贺梦子杨淑娟马淑梅
Owner JILIN UNIV
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