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A method for tissue culture of Trumpetella pseudophylla

A technology of trumpet algae and tissue culture, applied in the field of plant tissue culture, can solve the problems of low induction rate, not easy to kill, backward culture technology, etc., and achieve the effect of promoting redifferentiation

Active Publication Date: 2016-02-03
陆俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tissue culture technology of marine plants is relatively backward. The main reason is that it is impossible to obtain a large number of sterile and viable explants. Seaweed is mainly composed of cells. Although the structure of large seaweed is relatively complex, the type and dosage of disinfectants The effect of explants is very significant, high doses are easy to kill explants, and low doses are not easy to kill bacteria or fungi that enter the interior of seaweed cells
Moreover, the type of medium and the ratio of hormones in marine plant tissue culture are still in the exploratory stage, so the plant tissue culture technology of S. pseudophyllum is still relatively backward, and it is difficult to induce callus, buds or roots, or the induction rate is extremely high. Low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Use the rhizoid of Trumpetella pseudophyllum as explants, use a hairbrush to remove the algae and other attachments attached to the explants, and then soak the explants in 2% streptomycin for 3 hours. Then take out the explants, sterilize in 1% povidone-iodine for 2 minutes, then take out the explants and sterilize them in 2% sodium hypochlorite solution for 3 minutes, and finally rinse them with cooled filtered high-pressure sterilized sea water ; Cut the rhizoid explants into sections with a length of 0.2-0.5cm in the sterile operating table, and inoculate the sections into the culture medium, inoculate 5 sections in each culture dish, and inoculate 100 sections in total ; shaker culture, the speed of the shaker is 80 ~ 100r / min, the culture conditions are light intensity 4000 ~ 5000Lx, light time 12h, temperature 18 ~ 22 ℃; the formula of the medium is 1LPES medium with NAA0.5mg, KT0 .5mg, ascorbic acid 5mg, sucrose 30g; cultivated for 4 weeks until regeneration buds...

Embodiment 2

[0021] Use the rhizoid of Trumpetella pseudophyllum as explants, use a hairbrush to remove the algae and other attachments attached to the explants, and then soak the explants in 2% streptomycin for 3 hours. Then take out the explants, sterilize in 1% povidone-iodine for 2 minutes, then take out the explants and sterilize them in 2% sodium hypochlorite solution for 3 minutes, and finally rinse them with cooled filtered high-pressure sterilized sea water ; Cut the rhizoid explants into sections with a length of 0.2-0.5cm in the sterile operating table, and inoculate the sections into the culture medium, inoculate 5 sections in each culture dish, and inoculate 100 sections in total ;Shaker culture, the speed of the shaker is 80-100r / min, the culture conditions are light intensity 4000-5000Lx, light time 12h, temperature 18-22 ℃; the formula of the medium is 1LPES medium with NAA4mg, KT2mg, ascorbic acid 10mg, 30g of sucrose; cultivated for 4 weeks until the regeneration buds for...

Embodiment 3

[0023] Use the rhizoid of Trumpetella pseudophyllum as explants, use a hairbrush to remove the algae and other attachments attached to the explants, and then soak the explants in 2% streptomycin for 3 hours. Then take out the explants, sterilize in 1% povidone-iodine for 2 minutes, then take out the explants and sterilize them in 2% sodium hypochlorite solution for 3 minutes, and finally rinse them with cooled filtered high-pressure sterilized sea water ; Cut the rhizoid explants into sections with a length of 0.2-0.5cm in the sterile operating table, and inoculate the sections into the culture medium, inoculate 5 sections in each culture dish, and inoculate 100 sections in total Shaker culture, the speed of the shaker is 80-100r / min, the culture conditions are light intensity 4000-5000Lx, light time 12h, temperature 18-22℃; the formula of the medium is 1LPES medium with NAA4mg and KT0.5mg added , 7 mg of ascorbic acid, 30 g of sucrose; cultivated for 4 weeks until the formati...

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Abstract

The invention discloses a tissue culture method of turbinaria conoides. Rhizoid of the turbinaria conoides is taken as an explant and inoculated in an induction medium to induct generation of regeneration buds after disinfected by streptomycin, povidone iodine and sodium hypochlorite, a PES (Provasoli's enriched seawater) culture medium is taken as a basic culture medium, and 0.5-4 mg of NAA (naphthalene acetic acid), 0.5-2 mg of KT (kinetin), 5-10 mg of ascorbic acid and 30 g of cane sugar are added into the PES culture medium with a volume of 1 L. The rhizoid of the turbinaria conoides is taken as the explant, streptomycin, povidone iodine and sodium hypochlorite are matched for use, and an abundant sterile explant can be obtained; and the PES culture medium is taken as the basic culture medium, and the rhizoid explant is effectively prompted to regenerate after NAA and KT are added, so that a large quantity of regeneration buds are generated, and the inductivity is up to 81%.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of Trumpetella pseudophylla. Background technique [0002] Pseudomonas microphyllum ( Turbinaria conoides ) belongs to the Phaeophyta, Fucusaceae, Sargassumaceae, and Trumpetella genus, and is a common large brown algae in my country. Trumpetella microphylla is rich in marine steroids such as marine sterols. Compared with sterols in terrestrial plants, marine sterols have more abundant skeletons and branched chains. This unique structure endows it with anti-tumor and anti-viral properties. , anti-inflammatory and bactericidal special effects, and can be used to prepare enzyme inhibitors, therefore, marine steroids have become a class of ingredients that attract attention in the medical and pharmaceutical industries. [0003] At present, the wild resources of Trumpetella pseudophylla are mainly used, but the wild resources are not abundan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张明
Owner 陆俊
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