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Litchi R2R3-MYB gene LcMYB1 and application thereof

A R2R3-MYB, gene technology, applied in application, genetic engineering, plant genetic improvement and other directions

Inactive Publication Date: 2014-04-16
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the regulatory genes related to litchi anthocyanin synthesis and metabolism.

Method used

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  • Litchi R2R3-MYB gene LcMYB1 and application thereof
  • Litchi R2R3-MYB gene LcMYB1 and application thereof
  • Litchi R2R3-MYB gene LcMYB1 and application thereof

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Experimental program
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Effect test

Embodiment 1

[0032] 1. Litchi peel selection

[0033] The lychee invented separately is selected from the "Nuomici" lychee, and the skin, pulp, leaves, and stems of the "Nuomici" lychee used are taken from the Changgang Mountain Orchard of South China Agricultural University. The developmental stage samples of ‘Nuomici’ were collected at 52 days, 59 days, 62 days, 66 days and 73 days after flowering. Use a 0.9cm caliber puncher to take out the peel discs, put them into a tin foil bag and quickly freeze them with liquid nitrogen, take them back to the laboratory and store them in a -80°C refrigerator for determination of anthocyanin content in the peel, gene cloning and gene expression analyze. ‘Nuomici’ lychee peel, pulp, leaves, and stems were collected from the Changgang Mountain Orchard of South China Agricultural University.

[0034] 2. Extraction and detection of RNA from litchi peel

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Abstract

The invention discloses a litchi R2R3-MYB gene LcMYB1 and an application thereof, and belongs to the field of biological genes. A nucleotide sequence of a gene LcMYB1 is obtained by designing a LcMYB1 segment, a RACE segment and an overall-length primer in an amplifying and sequencing mode; the LcMYB1 is analyzed to possibly have the function of regulating and controlling the biosynthesis of anthocyanins of a litchi peel from the amino acid structure of the LcMYB1 by translating the nucleotide sequence into an amino acid sequence and analyzing the structure of the gene; the LcMYB1 is verified to have the function of regulating and control the biosynthesis of the anthocyanins via the transformation of tobaccos. Thus, the foundation for the application of the LcMYB1 for improving the accumulation of plant pigments is provided.

Description

technical field [0001] The invention belongs to the field of biological genes, in particular to a litchi R2R3-MYB gene LcMYB1 and its application. Background technique [0002] The color performance of most flowers and fruits and vegetables is mainly due to the accumulation of anthocyanins, and the content of anthocyanins is an important aspect that determines the quality of flowers, fruits and vegetables. Due to the antioxidant properties of anthocyanins, more people like red to purple vegetables and fruits. In addition to bringing rich and bright colors to vegetables, flowers, and fruits, anthocyanins also have protective effects on plants from biotic and abiotic stresses, such as: ultraviolet radiation, cold stress, and drought stress responses (Castellarin et al.2007; Ubi et al.2006; Zhang et al.2012). Recent studies at home and abroad have shown that the regulation of anthocyanin anabolism is at the transcriptional level, mainly by regulating the expression of structu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/84A01H5/00
Inventor 胡桂兵赖彪王惠聪秦永华黄旭明张春阳
Owner SOUTH CHINA AGRI UNIV
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