Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Indirect Competitive ELISA Detection Method for (2-Ethylhexyl) Phthalate

A phthalic acid and enzyme-linked immunosorbent assay technology, which is used in measurement devices, biological tests, material inspection products, etc., to achieve good accuracy and reliable results.

Active Publication Date: 2015-10-21
武汉菲恩生物科技有限公司
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide an indirect competitive ELISA detection method for (2-ethylhexyl) phthalate, to solve the problem that (2-ethylhexyl) phthalate is a hapten and cannot directly act on it. Problems with an organism that makes it produce antibodies

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0075] The following is a preferred example among various implementations of the present invention.

[0076] The indirect competitive enzyme-linked immunoassay for the determination of (2-ethylhexyl) phthalate has good specificity, high sensitivity, and is convenient and rapid. Antigen-antibody reaction to detect (2-ethylhexyl) phthalate. First, 4-nitrophthalate (2-ethylhexyl) ester was synthesized from 4-nitrophthalic acid, and then reduced to obtain the hapten 4-aminophthalate (2-ethylhexyl) ester, The immune antigen is prepared by coupling the BSA, and the immune antigen is injected into the animal body, and the animal grows and reacts to obtain the antibody after a certain period of time. A method for the determination of (2-ethylhexyl) phthalate was established through the specific reaction among antigen, antibody and enzyme-labeled secondary antibody.

[0077] 1. Experimental drugs and instruments

[0078] (1) Drugs

[0079] 4-nitrophthalic acid, thionyl chloride, zi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to an indirect competive enzyme-linked immunosorbent assay detection method of bis(2-ethylhexyl)phthalate, wherein the indirect competive enzyme-linked immunosorbent assay belongs to the luminosity enzyme-linked immunosorbent assay, the specific antigen-antibody reaction is adopted to efficiently and rapidly detect the environmental hormone bis(2-ethylhexyl)phthalate. According to the present invention, 4-amino bis(2-ethylhexyl)phthalate easily binding with protein is synthesized, protein is bond with the 4-amino bis(2-ethylhexyl)phthalate to prepare immunogen, the immunogen is injected into rabbits to obtain antibody, and the indirect competive enzyme-linked immunosorbent assay detection method is established to detect the bis(2-ethylhexyl)phthalate; and the bis(2-ethylhexyl)phthalate is the environmental hormone harmful for organism health, and the bis(2-ethylhexyl)phthalate content in river, lakes, food, cosmetics and other articles can be efficiently and rapidly detected with the establishment of the indirect competive enzyme-linked immunosorbent assay detection method.

Description

technical field [0001] The invention relates to an indirect competitive ELISA detection method for phthalate (2-ethylhexyl) ester. Background technique [0002] Phthalates are mainly used in polyvinyl chloride materials to change polyvinyl chloride from hard plastic to elastic plastic and act as plasticizers. It is commonly used in hundreds of products such as toys, food packaging materials, medical blood bags and hoses, vinyl flooring and wallpaper, cleaners, lubricants, personal care products, and more. Since phthalates are added to the plastic production process as plasticizers and do not combine with plastic molecules, such substances can seep out of plastic products, and humans can pass through the skin during the use of plastic products. Contact, inhalation, and direct ingestion of such substances can cause great harm to human health. [0003] Phthalates play a role similar to estrogen in the human body and animals, which can interfere with endocrine, reduce the amou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558G01N33/535
CPCG01N21/31G01N33/53G01N33/535G01N33/558
Inventor 张明翠钟君亚
Owner 武汉菲恩生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com