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Preparation and use of eg and tcm199 as bovine semen diluent and freezing solution

A TCM199, the technology of the preparation method, applied in the application, preservation of human or animal body, animal husbandry, etc., can solve the problems of large molecular weight of refrigerant, complicated operation technology, large particles, etc., achieve simple method, improve conception rate, reduce The effect of small particles

Inactive Publication Date: 2017-01-18
GANSU ANIMAL HUSBANDRY & VETERINARY MEDICINE INST
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Problems solved by technology

[0008] The purpose of the embodiments of the present invention is to provide a method for preparing and using EG and TCM199 as a bovine semen diluent and freezing liquid, aiming at solving the problem that the existing bovine semen diluent has large particles and high viscosity; the molecular weight of the refrigerant is large, Strong toxicity; technically complex issues

Method used

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  • Preparation and use of eg and tcm199 as bovine semen diluent and freezing solution
  • Preparation and use of eg and tcm199 as bovine semen diluent and freezing solution
  • Preparation and use of eg and tcm199 as bovine semen diluent and freezing solution

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Embodiment Construction

[0029] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0030] The application principle of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0031] like figure 1 Shown, the preparation method of the bovine semen dilution liquid of the embodiment of the present invention comprises the following steps:

[0032] S101: Accurately weigh 2 grams of TCM199, 40 grams of trishydroxyaminomethane, 20 grams of citric acid, 0.1 grams of sodium pyruvate, 5 grams of fructose, and 1 gram of 800,000 units of penicillin sodium with an electronic balance;

[0033] S102: Put the above components into a 1000 ml volumet...

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Abstract

The invention discloses a preparation method and a use method for EG (ethylene glycol) and TCM199 serving as bovine semen diluent and bovine semen freezing liquid. The preparation method for the EG and TCM199 serving as the bovine semen diluent and the bovine semen freezing liquid comprises the step of preparing the bovine semen diluent and the bovine semen freezing liquid. The use method for the EG and TCM199 serving as the bovine semen diluent and the bovine semen freezing liquid comprises the following steps of preparing the bovine semen diluent and the bovine semen freezing liquid; collecting semen; performing primary dilution; performing secondary dilution; performing freezing storage. According to the preparation method and the use method, the defreezing activity of frozen bovine semen is improved; the bovine artificial insemination conception rate is increased, the viscosity of the bovine semen diluent is lowered, and particles of the bovine semen diluent are reduced; a new-generation freezing agent with a small molecular weight and low toxicity is adopted to replace the original freezing agent with a large molecular weight and high toxicity; the operation is simplified; the preparation method and the use method are suitable for various types of large-scale production conditions.

Description

technical field [0001] The invention belongs to the technical field of bovine artificial insemination, and in particular relates to a method for preparing and using EG and TCM199 as bovine semen diluent and freezing liquid. Background technique [0002] Cattle breeding methods include self-crossing and artificial insemination. Artificial insemination is divided into fresh mating and frozen mating. Remote and centralized artificial insemination using thin tube frozen semen is the most important method of cattle breeding. Therefore, the quality of the frozen sperm is the key link to ensure the conception rate of artificial insemination. The quality of straw frozen semen is affected by the health status of the donor bull, feeding level, semen quality, production process of straw frozen semen and storage conditions. In these links, except for the production of straw frozen semen, manual control can be carried out, while the straw The production process of frozen essence is grea...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 桑国俊邱忠玉郭海龙王珂容维中保国俊
Owner GANSU ANIMAL HUSBANDRY & VETERINARY MEDICINE INST
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