Collecting device and method of root exudates from xerophyte

A technology of root exudates and xerophytes, applied in the field of xerophyte root exudates collection devices, can solve the problem of inability to collect root exudates, not suitable for collection of xerophyte root exudates, and limit xerophyte root exudates research and other issues, to achieve the effect of water infiltration, no damage to the rhizosphere environment, and collection

Inactive Publication Date: 2014-05-07
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

This device can neither destroy the natural growth condition between the root system and the soil, but also can better select the collection site, but it cannot collect the root exudates of tubers or tu

Method used

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  • Collecting device and method of root exudates from xerophyte

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Embodiment Construction

[0028] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0029] Such as figure 1 As shown, the culture device is a plexiglass bucket 2 with a frosted inner wall, the filling culture medium is a mixture 3 of soil and vermiculite, and the research object is a plant 1 to be tested. The device used for irrigation is a specially treated PVC pipe 4, the plexiglass barrel is filled with washed sand 5, and the stem of the barrel is filled with non-woven fabric 6. Use iron clip 7 to control the collection of root exudates, and the other end of rubber tube 8 is connected with collecting device and extraction syringe 9.

[0030] Collection of root exudates of Rehmannia glutinosa:

[0031] Firstly, a custom-made bottomless internal frosted plexiglass barrel with a height of 40cm, a thickness of 0.2cm, a barrel body of 28cm, a diameter of 21cm, a barrel shoulder of 4cm, and a barrel stem of 8cm in dia...

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Abstract

The invention provides a collecting device of root exudates from xerophytes. The collecting device comprises a cultivating bucket, wherein the bottom surface of the cultivating bucket is communicated with a rubber tube, and the other end of the rubber tube is connected with an injector, a soil vermiculite mixed culture medium layer and a filter layer are sequentially spread in the cultivating bucket from top to bottom, and an irrigation tube is further arranged in the cultivating bucket. The invention also provides a collecting method for the root exudates from xerophytes, which is designed according to the collecting device. The collecting device and method are suitable for research on plants under soil cultivation condition, especially the root exudates from xerophytes; compared with water-culture or simple medium cultivation method, the method provided by the invention has the advantages that the actual condition of the root exudates from xerophytes under the natural condition can be better reflected; meanwhile, the collecting device cannot disturb the root growth or damage the rhizoapple environment in the premise of guaranteeing the normal growth of the plant, and the root damage and bleeding disturbance caused by collecting the root exudates through a traditional earth culture method are avoided.

Description

technical field [0001] The invention relates to a device for collecting xerophyte root exudates and a collecting method thereof. Background technique [0002] At present, the collection of root exudates mainly includes hydroponics, matrix culture collection, and soil culture. Hydroponics does not require special collection devices, but hydroponic plants generally have less root hair development and no mechanical resistance, which is quite different from the aeration, nutrient distribution, and root branching structure grown under real soil culture conditions. Therefore, hydroponics cannot obtain the root exudates of xerophytes. The matrix culture method can ensure the ventilation of the root system and has a certain mechanical resistance, but it is quite different from the root exudates in the natural state. [0003] The traditional soil culture method mainly adopts two methods to collect root exudates. One is to take out the root system of the soil culture plants after gr...

Claims

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Application Information

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IPC IPC(8): G01N1/14
Inventor 张重义张宝尤垂淮李晶晶冯法节曹晓风龚文荣古力王建明
Owner FUJIAN AGRI & FORESTRY UNIV
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