Injectable modified hyaluronic acid and its preparation method and composition
A hyaluronic acid and modification technology, applied in the field of modified hyaluronic acid, can solve the problems of host poisoning and achieve the effect of simple and efficient method and mild reaction conditions
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[0074] The preparation method of modified hyaluronic acid comprises the following steps:
[0075] 1) Dissolving hyaluronic acid in an aqueous solution, adding 1-amino-3,3-diethoxypropane ADEP and an optional amide reaction catalyst, reacting completely to obtain the reactant HA-ADEP and purifying;
[0076] 2) Dissolve the purified HA-ADEP, adjust the pH of the HA-ADEP solution to acidic with a non-oxidizing acid, complete the reaction, and purify to obtain modified HA, which is denoted as AHA.
[0077] The reaction formula is as follows:
[0078] .
[0079] The amount of ADEP added can be adjusted according to the substitution ratio. In order to speed up the reaction and facilitate the subsequent purification, the amount of ADEP added is generally added in excess.
[0080] The above-mentioned aqueous solution can be water, or various buffers, such as PBS solution, acetate buffer, TE buffer, HEPES buffer, etc.; HA and ADEP are dissolved in the aqueous solution, which can re...
Embodiment 1
[0085] Dissolve 250mg HA in 10ml of 0.1mol / l PBS, add 125mgEDC and 100mgNHS as amide reaction catalyst, stir evenly, then add 389mgADEP and let it stand to react overnight, dialyze to remove unreacted small molecules, dry to obtain purified reactant HA-ADEP ;
[0086] Dissolve HA-ADEP in water, add hydrochloric acid to adjust the pH of the solution to 1-3, let it stand for reaction to form aldehyde groups, after the reaction is complete, dialyze and purify, and dry to obtain modified HA, which is designated as AHA.
Embodiment 2
[0088] Dissolve 250 mg HA in 10 ml of 0.1 mol / l acetic acid, add 100 mg EDC and 100 mg N-hydroxysulfosuccinimide as amide reaction catalysts, stir evenly, then add 800 mg ADEP and let it stand for reaction overnight, dialyze to remove unreacted small molecules, dry Obtain purified reactant HA-ADEP;
[0089] Dissolve HA-ADEP in water, add hydrochloric acid to adjust the pH of the solution to 1-3, let it stand for reaction to form aldehyde groups, after the reaction is complete, dialyze and purify, and dry to obtain modified HA, which is designated as AHA.
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