Rat pancreas islet epithelioid stem cell system

A stem cell line and stem cell technology, applied in the direction of animal cells, vertebrate cells, artificial cell constructs, etc., can solve the problem that there is no established rat pancreatic islet-like stem cell line

Inactive Publication Date: 2014-06-25
GUANGDONG OCEAN UNIVERSITY
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  • Description
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Problems solved by technology

At present, there is no report on the establishment of r

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  • Rat pancreas islet epithelioid stem cell system
  • Rat pancreas islet epithelioid stem cell system
  • Rat pancreas islet epithelioid stem cell system

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Embodiment 1

[0047] 1. Morphological characteristics of rat pancreatic endothelial-like stem cells

[0048] H·E staining: after the rat pancreatic epithelial-like stem cells were fully adhered to the wall, they showed polygonal epithelial-like ( figure 1 ). The staining method refers to Situ Zhenqiang, Wu Junzheng. Cell Culture [M]. World Book Publishing Company. 2007. Slightly;

[0049] Giemsa staining: the nuclei of rat pancreatic endothelial-like stem cells are oval, and the nucleoli are mostly single or double nucleoli ( figure 2 ). For the staining method, refer to R.I. Frescheini. Animal Cell Culture—Basic Technical Guidelines [M]. Science Press. 2008. Slightly;

[0050] 2. Growth characteristics of rat pancreatic endothelial-like stem cells

[0051] The clonal growth of rat pancreatic endothelial-like stem cells; the growth curve showed that the cells grew slowly on the 1st day after inoculation, entered the logarithmic growth phase on the 2nd to 4th day, reached the plateau st...

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Abstract

The invention relates to a rat pancreas islet epithelioid stem cell system and belongs to the field of animal cell (tissue) engineering. The rat pancreas islet epithelioid stem cell is from a normal adult rat pancreas islet tissue, cultured in vitro, and can grow in a clonal manner; moreover, the cell has a plurality of differentiation potentials, specifically cell adherent culture is polygonal and epithelioid, cell nucleus is oval, and nucleolus is a single nucleolus or amphinucleolus; in cell clonal growth, cells grow slowly on the first day after being inoculated, enter a logarithm growth period on the second day to the fourth day and enter a platform period on the fifth day to the sixth day, and multiplication capacity is reduced on the seventh day; the cells are normal diploid cells with a chromosome number 2n equal to 42; an octamer transcription factor 4, a paired-gene cassette 4, paired-gene cassette 6, a neurogenic factor 3 and nidogen are expressed on protein level. The rat pancreas islet epithelioid stem cell system has the plurality of differentiation potentials, is directionally induced in vitro to differentiate and form nerve cells, fat cells and osteoblast, and is free of oncogenicity.

Description

technical field [0001] The present invention relates to human and other mammalian islet stem cell lines and their characteristics, in particular to a rat pancreatic islet-like stem cell line and its morphology, growth, expression and differentiation characteristics, and belongs to the field of animal cell (tissue) engineering. Background technique [0002] Diabetes mellitus (DM) is one of the major diseases that endanger human health. According to statistics, the number of diabetic patients in the world has reached 346 million (Am J Stem Cells.2012,1(3):196-204.), while the traditional Drugs and insulin replacement therapy can not fundamentally cure the disease. In the past ten years, with the development of transplantation technology, pancreatic islet transplantation has achieved certain curative effect in the treatment of diabetes (N Engl J Med.2000,343(4):230-238. Diabetes.2005,54(7):2060-2069. Am J Transplant. 2008,8(11):2463-2470. Immune Netw. 2013,13(6):235-239.). Ho...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12R1/91
Inventor 安立龙效梅
Owner GUANGDONG OCEAN UNIVERSITY
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