Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rat pancreas islet epithelioid stem cell system

A stem cell line and stem cell technology, applied in the direction of animal cells, vertebrate cells, artificial cell constructs, etc., can solve the problem that there is no established rat pancreatic islet-like stem cell line

Inactive Publication Date: 2014-06-25
GUANGDONG OCEAN UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the establishment of rat pancreatic endothelial-like stem cell lines in China.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rat pancreas islet epithelioid stem cell system
  • Rat pancreas islet epithelioid stem cell system
  • Rat pancreas islet epithelioid stem cell system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Morphological characteristics of rat pancreatic endothelial-like stem cells

[0048] H·E staining: after the rat pancreatic epithelial-like stem cells were fully adhered to the wall, they showed polygonal epithelial-like ( figure 1 ). The staining method refers to Situ Zhenqiang, Wu Junzheng. Cell Culture [M]. World Book Publishing Company. 2007. Slightly;

[0049] Giemsa staining: the nuclei of rat pancreatic endothelial-like stem cells are oval, and the nucleoli are mostly single or double nucleoli ( figure 2 ). For the staining method, refer to R.I. Frescheini. Animal Cell Culture—Basic Technical Guidelines [M]. Science Press. 2008. Slightly;

[0050] 2. Growth characteristics of rat pancreatic endothelial-like stem cells

[0051] The clonal growth of rat pancreatic endothelial-like stem cells; the growth curve showed that the cells grew slowly on the 1st day after inoculation, entered the logarithmic growth phase on the 2nd to 4th day, reached the plateau st...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a rat pancreas islet epithelioid stem cell system and belongs to the field of animal cell (tissue) engineering. The rat pancreas islet epithelioid stem cell is from a normal adult rat pancreas islet tissue, cultured in vitro, and can grow in a clonal manner; moreover, the cell has a plurality of differentiation potentials, specifically cell adherent culture is polygonal and epithelioid, cell nucleus is oval, and nucleolus is a single nucleolus or amphinucleolus; in cell clonal growth, cells grow slowly on the first day after being inoculated, enter a logarithm growth period on the second day to the fourth day and enter a platform period on the fifth day to the sixth day, and multiplication capacity is reduced on the seventh day; the cells are normal diploid cells with a chromosome number 2n equal to 42; an octamer transcription factor 4, a paired-gene cassette 4, paired-gene cassette 6, a neurogenic factor 3 and nidogen are expressed on protein level. The rat pancreas islet epithelioid stem cell system has the plurality of differentiation potentials, is directionally induced in vitro to differentiate and form nerve cells, fat cells and osteoblast, and is free of oncogenicity.

Description

technical field [0001] The present invention relates to human and other mammalian islet stem cell lines and their characteristics, in particular to a rat pancreatic islet-like stem cell line and its morphology, growth, expression and differentiation characteristics, and belongs to the field of animal cell (tissue) engineering. Background technique [0002] Diabetes mellitus (DM) is one of the major diseases that endanger human health. According to statistics, the number of diabetic patients in the world has reached 346 million (Am J Stem Cells.2012,1(3):196-204.), while the traditional Drugs and insulin replacement therapy can not fundamentally cure the disease. In the past ten years, with the development of transplantation technology, pancreatic islet transplantation has achieved certain curative effect in the treatment of diabetes (N Engl J Med.2000,343(4):230-238. Diabetes.2005,54(7):2060-2069. Am J Transplant. 2008,8(11):2463-2470. Immune Netw. 2013,13(6):235-239.). Ho...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/071C12R1/91
Inventor 安立龙效梅
Owner GUANGDONG OCEAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com