Method for determining residual sedative type veterinary medicaments in mutton

A technology for sedatives and veterinary drugs, applied in the field of simultaneous determination of multiple sedative drugs in meat

Inactive Publication Date: 2014-07-30
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The national standard stipulates that liquid-mass spectrometry is used for the detection of such drug

Method used

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  • Method for determining residual sedative type veterinary medicaments in mutton
  • Method for determining residual sedative type veterinary medicaments in mutton
  • Method for determining residual sedative type veterinary medicaments in mutton

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Embodiment 1: a kind of method for measuring residual sedative class veterinary drug in mutton, its main feature is to comprise the following steps:

[0077] The residual sedative veterinary drugs are zolpidem, haloperidol, chlordiazepoxide, promethazine hydrochloride, nitrazepam, chlorpromazine hydrochloride, perphenazine, fluphenazine hydrochloride, clonazepam, Xylazine hydrochloride, propionpromazine, carbazolol, acepromazine, droperidol, azaperone;

[0078] (1) The mutton was minced, mixed and homogenized, and stored at -40°C for later use.

[0079] (2) Take out the spare sample and thaw it to room temperature; accurately weigh 5.5g mutton sample into a 50mL polypropylene centrifuge tube, add 15mL ammonia water-acetonitrile (10:90, v / v) and 30μL β-glucuronidase, place in In the dark, vortex and shake at 40°C for 12 hours, ultrasonically extract at 40°C for 15min, centrifuge at 4500r / min for 10min, remove the supernatant, add 15mL of ammonia-acetonitrile (10:90, v / v...

Embodiment 2

[0089] Embodiment 2: a kind of method for measuring residual sedative class veterinary drug in mutton, its main feature is to comprise the following steps:

[0090] The residual sedative veterinary drugs are zolpidem, haloperidol, chlordiazepoxide, promethazine hydrochloride, nitrazepam, chlorpromazine hydrochloride, perphenazine, fluphenazine hydrochloride, clonazepam, Xylazine hydrochloride, propionpromazine, carbazolol, acepromazine, droperidol, azaperone;

[0091] (1) The mutton was minced, mixed and homogenized, and stored at -18°C for later use.

[0092] (2) Take out the spare sample and thaw it to room temperature; accurately weigh 4.5g mutton sample into a 50mL polypropylene centrifuge tube, add 15mL ammonia water-acetonitrile (10:90, v / v) and 30μL β-glucuronidase, place in In the dark, vortex and shake at 35°C for 8 hours, ultrasonically extract at 30°C for 10 minutes, centrifuge at 3500r / min for 5 minutes, remove the supernatant, add 15mL ammonia water-acetonitrile ...

experiment example

[0100] Mutton samples were purchased from the market (Lanzhou), minced, mixed, homogenized, and stored at -18°C for later use. Control products Zolpidem tartrate (Lot: 171258-200601, 100%), haloperidol (Lot: 100313-200301, 100%), promethazine hydrochloride (Lot: 100422-201002, 99.4%), chlordiazepoxide (Lot: 171248-200301, 100%), Chlorpromazine Hydrochloride (Lot: 100460-200501, 100%), Perphenazine (Lot: 100133-200602, 100%), Fluphenazine Hydrochloride (Lot: 100162- 201003, 98.8%), nitrazepam (Lot: 171217-200402, 100%), clonazepam (Lot: 171227-201103, 100%), etc. were purchased from China Institute for the Control of Pharmaceutical and Biological Products; xylazine hydrochloride (Lot : 11010, 98.0%), propionpromazine (Lot: 10404, 99.0%), carbazolol (Lot: 00421, 99.0%), azapirone (Lot: 20124, 98.5%), acepromazine (Lot: 20124, 98.5%), acepromazine (Lot: : 21105, 99%) etc. are Dr.Ehrenstorfer GmbH company standard items (Germany); Droperidol (CDCU-1229001) is USP standard items. ...

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Abstract

The invention relates to a method for determining residual medicaments in mutton, and in particular relates to a method for determining multiple sedative type medicaments in mutton at the same time. The residual sedative type veterinary medicaments refer to zolpidem, haloperidol, chlordiazepoxide, promethazine, nitrazepam, chlorpromazine hydrochloride, perphenazine, fluphenazine hydrochloride, clonazepam, xylazine hydrochloride, propionylpromazine, carazolol, acepromazine, droperidol and azaperone. The method has the advantage that the residual amounts of 15 types of sedative type medicaments in mutton are determined through high-resolution liquid chromatography-tandem mass spectrometry. The method is high in sensitivity and high in recovery rate, and can meet the detection requirements on veterinary medicaments.

Description

technical field [0001] The invention relates to a method for detecting residual drugs in meat, in particular to a method for simultaneously measuring multiple sedative drugs in meat. Background technique [0002] Sedatives refer to a class of drugs that can slightly inhibit the central nervous system and weaken functional activities, thereby eliminating restlessness and restoring tranquility. In recent years, driven by economic interests, individual livestock breeders have added such drugs in the process of raising livestock and poultry without authorization to play the role of sedation and hypnosis, weight gain and fattening, and shortening the time for slaughter; in addition, in the process of animal transportation, for To reduce animal death and weight loss, to prevent the reduction of meat quality, these drugs are also often used to reduce the loss caused by stress. Illegal use of such drugs will inevitably leave their original form and metabolites in food of animal ori...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 魏晋梅罗玉柱周围解迎双刘秀张丽
Owner GANSU AGRI UNIV
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