Detection method for residual amount of cyflufenamid in wheat

Abstract

The invention discloses a detection method for a residual amount of cyflufenamid in wheat. The method comprises the following steps: 1, preparing a standard solution; 2, drawing a standard curve; 3, extracting a sample; 4, purifying the sample; 5, detecting the sample; and 6, calculating a result. According to the detection method, acetonitrile is used as an extraction liquid, compared with the acetone serving as the extraction liquid, less liquid is used, and the later purification is relatively simple; compared with the methanol serving as the extraction liquid, after the extraction and salt precipitation, a heating plate is dried in a steaming manner, and no rotary evaporation is needed, so that the working efficiency is improved; the popularization of the instrument for detection is relatively high, and the method is easy to popularize and use; the reproducibility, the accuracy, the precision and the detection limit of the detection method of the invention can meet the requirements on the analysis of residues of pesticide in the wheat; and by adopting the detection method, the environmental pollution is reduced, the detection cost is reduced, the working efficiency is improved, the practicability is high, and the popularization and application value is high.

Description

technical field [0001] The invention belongs to the field of pesticide residue detection, and in particular relates to a method for detecting cyflufenamide residues in wheat. Background technique [0002] English name: cyflufenamid, CAS number: 180409 -60 -3, molecular formula: C 20 H 17 F 5 N 2 O 2 , chemical name: (Z)-N-[α-(cyclopropylmethoxyimino)-2,3-difluoro-6-(trifluoromethyl)benzyl]-2-phenylacetamide. Cyflufenamide is an amide fungicide, which inhibits the formation and growth of haustoria, secondary hyphae and attachments on the hyphae of the powdery mildew fungus in the life cycle (ie, the disease process), but it does not affect the formation of spores. Germination, bud tube length and attachment formation had no effect. It has no cross-resistance with the currently commercialized and under-study fungicides, and is effective against all strains of powdery mildew including resistance to demethylation inhibitors, sterols and benzimidazole fungicides. It has pr...

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Problems solved by technology

[0003] In the extraction methods in the prior art, the extracting solution is mostly acetone, which requires a large amount, complicated purification in the later stage, and serious environmental pollution; after extraction and salting out, it is mostly rotary evaporation, and the work efficiency is low; some methods are simple in pretreatment, but the detection method used The instrument is mass spectrometry, the popularity rate is low, the method is difficult to popularize and apply, and the detection cost is high

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