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Orchid rapid-propagation culture medium formula

A medium formula and a technology for multiplying medium, which are applied in the field of medium formula for rapid propagation of orchid plant tissue culture, can solve the problems of difficult germination, high transplant mortality, and lack of fully developed embryos, etc. The effect of strong adaptability, high transplant survival rate and healthy tissue culture seedlings

Active Publication Date: 2014-08-27
INST OF DAFENG MARINE IND NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional reproduction method of orchids is branch reproduction, which has low reproduction coefficient and slow speed. Due to the limitation of geographical environment and seasons, it is difficult to achieve the purpose of rapid and efficient reproduction. Does not have a fully developed embryo, which is difficult to germinate
Tissue culture is an effective solution to the reproduction of orchid plants at present, but most of the medium used is to add chemically synthesized plant hormones to promote the rapid reproduction and growth of plants, and the tissue culture seedlings often have poor stress resistance. , high mortality in transplanting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Tissue Culture Rapid Propagation of Dendrobium officinale

[0044] (1) Explants: fruit pods of Dendrobium officinale, the pods are complete and without cracks;

[0045] (2) Disinfection of explants: Wash the fruit pods with clean water, put them in 75% alcohol for 20 seconds, wash them twice with sterile water, then put the fruit pods in 0.1% mercuric chloride for 12 minutes, and wash them with sterile water for 5~ 7 times;

[0046](3) Inoculation and start-up culture: In a sterile environment, open a small opening on the side of the sterilized fruit pod, and sow the seeds into the sterile medium, that is, the start-up medium. The formula of the start-up medium is: Huabao No. 1 1.5g / L+ Huabao No. 2 1.0g / L+ peptone 0.5g / L+ potato 30g / L+ coconut milk 100ml / L+ sucrose 15g / L+ agar 5.5g / L, PH 6.0;

[0047] (4) Proliferation culture: 25~35 days after starting the culture, transfer the seedlings or protocorms of Dendrobium candidum to the proliferation medium. The formula of...

Embodiment 2

[0051] Rapid Propagation of Oncidium in Tissue Culture

[0052] (1) Explants: oncidium fruit pods, the pods are intact without cracks;

[0053] (2) Disinfection of explants: Wash the fruit pods with clean water, put them in 75% alcohol for 20 seconds, wash them twice with sterile water, then put the fruit pods in 0.1% mercuric chloride for 12 minutes, and wash them with sterile water for 5~ 7 times;

[0054] (3) Inoculation and start-up culture: In a sterile environment, open a small opening on the side of the sterilized fruit pod, and sow the seeds into the sterile medium, that is, sow the seeds into the sterile start-up medium, the formula of the medium For: Huabao No. 1 1.5g / L+ Huabao No. 2 1.0g / L+ peptone 0.5g / L+ potatoes 25g / L+ coconut milk 80ml / L+ sucrose 20g / L+ agar 5.0g / L, PH 5.8;

[0055] (4) Proliferation culture: 30 days after starting the culture, transfer oncidium seedlings or protocorms to the proliferation medium. The formula of the proliferation medium is: 1 / ...

Embodiment 3

[0059] Rapid Propagation of Bletilla striata Tissue Culture

[0060] (1) Explants: Bletilla striata fruit pods, the pods are complete and without cracks;

[0061] (2) Disinfection of explants: Wash the fruit pods with clean water, put them in 75% alcohol for 20 seconds, wash them twice with sterile water, then put the fruit pods in 0.1% mercuric chloride for 12 minutes, and wash them with sterile water for 5~ 7 times;

[0062] (3) Inoculation and start-up culture: In a sterile environment, open a small opening on the side of the sterilized fruit pod, and spread the seeds into the sterile medium. The formula of the medium is: Huabao No. 1 1.2g / L+flower Bao No. 2 1.5g / L+peptone 0.5g / L+potato 35g / L+coconut juice 120ml / L+sucrose 20g / L+agar 5.0g / L, PH 6.0;

[0063] (4) Proliferation culture: 25 days after starting the culture, transfer oncidium seedlings or protocorms to the proliferation medium. The medium formula is: 1 / 2MS+potato 30g / L+banana 55g / L+sucrose 25g / L+peptone 1.0g / ...

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Abstract

The invention relates to an orchid rapid-propagation culture medium formula. Culture mediums for fast propagation of orchids include starting culture mediums, proliferation culture mediums and rooting and seedling strengthening culture mediums which are used in three culture stages. According to the culture medium formula, no plant hormones are added, and the characteristic of wild imitation cultivation is achieved. Orchid tissue culture seedlings obtained through the formula are good in growth vigor, regular and high in transplantation survival rate.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a medium formula for rapid propagation of orchid plant tissue culture. Background technique [0002] Orchidaceae is a large family of angiosperms, and it is the largest family of higher plants except Asteraceae. According to statistics, there are about 800 genera and nearly 25,000 species of Orchidaceae known in the world, which are widely distributed except for two levels and extremes. Among the various terrestrial ecosystems outside the arid desert regions, the tropical regions are the most abundant. Traditional Chinese orchids mainly refer to plants of the genus Orchid, which are called Chinese orchids. Orchids, as a plant integrating ornamental and medicinal purposes, not only have a large number of fans, but also become an integral part of Chinese traditional culture. However, the traditional reproduction method of orchids is branch reproduction, which has ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张本厚胡燕花陈集双
Owner INST OF DAFENG MARINE IND NANJING UNIV OF TECH
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