Pseudomonas strain capable of producing siderophore with high yield and application thereof

A technology of Pseudomonas aeruginosa and siderophiles, applied in the direction of application, bacteria, fungicides, etc., can solve the problem of low biological effectiveness, achieve the effect of avoiding harm to the environment and promoting growth

Inactive Publication Date: 2014-09-03
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF2 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002]Iron is essential to the normal growth of most microorganisms, and generally requires 0.4-1.0μM iron to maintain thei

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pseudomonas strain capable of producing siderophore with high yield and application thereof
  • Pseudomonas strain capable of producing siderophore with high yield and application thereof
  • Pseudomonas strain capable of producing siderophore with high yield and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1. Screening of high-yield Pseudomonas siderophilus strains:

[0052] A total of 15 siderophilic strains were isolated from the MMSA-CAS plate, among which PA94 had the fastest color change. figure 1 After pouring the CAS blue medium on the MMSA plate of the PA94 strain shown, the blue around the colony began to become lighter at about 15 minutes, and turned orange-red at about 30 minutes, and then the color change circle became larger with time, and no longer expanded after 3 hours . Streak and purify 15 siderophilic-producing strains, inoculate a single colony in liquid MMSA medium, cultivate for 24 hours and centrifuge, and mix the supernatant with an equal volume of CAS solution, wherein PA94 strain As=OD 680 =0.174, control Ar=OD 680 =0.478 The maximum siderophilic activity unit, SU=63.54%, the ability to produce siderophilic As / Ar=0.36, ++++, the strain PA94 can produce a higher level of siderophilic.

[0053] 2. Identify the type of siderophiles produced by st...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a pseudomonas strain capable of producing siderophore with high yield and an application thereof. The serial number of the pseudomonas strain is PA94; the class name of the pseudomonas strain is pseudomonas aeruginosa; the collection number of the pseudomonas strain is CGMCCNo.9198; and the pseudomonas strain is collected in China General Microbiological Culture Collection Center which is located at No.3, Courtyard 1, Beichen west road, Chaoyang district, Beijing. Fifteen strains capable of producing the siderophore are obtained by screening soil samples collected from the Ji canal on an MMSA-CAS flat plate. The strain PA94 is cultivated in a MMSA culture medium for 24 hours, and tests prove that the activity unit SU of the siderophore is 63.54%, the As/Ar of siderophore is 0.36, and the siderophore production capacity of the strain is ++++. The produced siderophore is of a hydroxamic acid and citric acid mixed type, has an antagonistic effect on pathogenic bacteria and fungi, can be used for prevention and control of diseases in vegetable cultivation and is capable of promoting the growth of plants.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a strain of Pseudomonas bacteria with high siderophilic production and its application. Background technique [0002] Iron is crucial to the normal growth of most microorganisms, and generally requires 0.4-1.0 μM iron to maintain their normal growth. Although iron is abundant in the earth, it mostly exists in an insoluble form with extremely low bioavailability. Under such a stress, microorganisms have evolved multiple ways to obtain the necessary iron. Siderophore-mediated iron uptake is the most common way for microorganisms to obtain iron. Many microorganisms even synthesize and secrete siderogen beyond their own cell dry weight to meet the nutritional needs of iron. [0003] Siderophore is widely found in bacteria, fungi and plants. It is a relatively low molecular weight (500-1000D) iron chelating substance. It is synthesized under the condition of iron deficiency and has a str...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00C12R1/385
Inventor 王宏朱慧明张彦杨洪江
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap