Pseudomonas strain capable of producing siderophore with high yield and application thereof

Abstract

The invention relates to a pseudomonas strain capable of producing siderophore with high yield and an application thereof. The serial number of the pseudomonas strain is PA94; the class name of the pseudomonas strain is pseudomonas aeruginosa; the collection number of the pseudomonas strain is CGMCCNo.9198; and the pseudomonas strain is collected in China General Microbiological Culture Collection Center which is located at No.3, Courtyard 1, Beichen west road, Chaoyang district, Beijing. Fifteen strains capable of producing the siderophore are obtained by screening soil samples collected from the Ji canal on an MMSA-CAS flat plate. The strain PA94 is cultivated in a MMSA culture medium for 24 hours, and tests prove that the activity unit SU of the siderophore is 63.54%, the As / Ar of siderophore is 0.36, and the siderophore production capacity of the strain is ++++. The produced siderophore is of a hydroxamic acid and citric acid mixed type, has an antagonistic effect on pathogenic bacteria and fungi, can be used for prevention and control of diseases in vegetable cultivation and is capable of promoting the growth of plants.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a strain of Pseudomonas bacteria with high siderophilic production and its application. Background technique [0002] Iron is crucial to the normal growth of most microorganisms, and generally requires 0.4-1.0 μM iron to maintain their normal growth. Although iron is abundant in the earth, it mostly exists in an insoluble form with extremely low bioavailability. Under such a stress, microorganisms have evolved multiple ways to obtain the necessary iron. Siderophore-mediated iron uptake is the most common way for microorganisms to obtain iron. Many microorganisms even synthesize and secrete siderogen beyond their own cell dry weight to meet the nutritional needs of iron. [0003] Siderophore is widely found in bacteria, fungi and plants. It is a relatively low molecular weight (500-1000D) iron chelating substance. It is synthesized under the condition of iron deficiency and has a str...

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Problems solved by technology

[0002]Iron is essential to the normal growth of most microorganisms, and generally requires 0.4-1.0μM iron to maintain their normal growth, although iron is abundant in the earth , but mostly in insoluble form, with extremely low bioavailability

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