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Novel PCR (polymerase chain reaction) tube and preparation method thereof

A new and formulated technology, which is applied in the field of PCR experiment consumables, can solve the problems of poor transparency and easy cracking of PCR tubes, and achieve the effects of improving the success rate of experiments, increasing transparency, and facilitating observation

Inactive Publication Date: 2014-09-24
海南科晶生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention proposes a new type of PCR material, which solves the problems of poor transparency and easy cracking of existing PCR tubes

Method used

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  • Novel PCR (polymerase chain reaction) tube and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A new type of PCR tube, the formula includes the following components: homopolymer injection molding grade PP material 10.0kg, transparent PP material 10.0kg, dibenzylidene sorbitol 0.01kg, silicone oil 0.01kg.

[0022] A preparation method for a novel PCR tube, comprising

[0023] (1) Weigh the homopolymer injection molding grade PP material and transparent grade PP material according to the formula, add dibenzylidene sorbitol and organic silicon oil, and mix them in a high-speed mixer to obtain a mixture;

[0024] (2) The mixture is added into an injection molding machine for molding to obtain the novel PCR tube of the present invention.

Embodiment 2

[0026] A new type of PCR tube. The formula includes the following components: 13.0kg of homopolymer injection grade PP material, 13.0kg of transparent grade PP material, 0.05kg of bis(p-methylbenzylidene) sorbitol, and 0.05kg of organic silicone oil.

[0027] A preparation method for a novel PCR tube, comprising

[0028] (1) Weigh the homopolymer injection molding grade PP material and transparent grade PP material according to the formula, add bis(p-methylbenzylidene) sorbitol and organic silicon oil, and mix them in a high-speed mixer to obtain a mixture;

[0029] (2) The mixture is added into an injection molding machine for molding to obtain the novel PCR tube of the present invention.

Embodiment 3

[0031] A new type of PCR tube, the formula includes the following components: 15.0kg of homopolymer injection grade PP material, 15.0kg of transparent grade PP material, 0.1kg of (3,4-dimethyldibenzylidene) sorbitol, and 0.1kg of silicone oil.

[0032] A preparation method for a novel PCR tube, comprising

[0033] (1) Weigh the homopolymer injection molding grade PP material and transparent grade PP material according to the formula, add (3,4-dimethyldibenzylidene) sorbitol and silicone oil, and mix them in a high-speed mixer to obtain a mixture;

[0034] (2) The mixture is added into an injection molding machine for molding to obtain the novel PCR tube of the present invention.

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Abstract

The invention discloses a novel PCR (polymerase chain react) tube. A formula comprises the following components in parts by weight: 20-30 parts of PP (polypropylene) materials, 0.01-0.1 part of a transparent agent, 0.01-0.1 part of diffusion oil, wherein the PP materials consist of homopolymerization injection-molding-level and transparent-level PP materials. The invention further discloses a preparation method of the novel PCR tube. According to the novel PCB tube and the preparation method, transparency of the finished product is increased, so that reactants and reaction degree in the tube can be conveniently observed, and experiment progress is controlled; moreover, the novel PCR tube is strengthened in flexibility and is not easy to break, so that requirements of PCR are satisfied, and an experimental success rate is improved.

Description

technical field [0001] The invention relates to the field of PCR experiment consumables, in particular to a novel PCR tube and a preparation method thereof. Background technique [0002] Polymerase Chain Reaction (PCR) is used to amplify specific DNA fragments. It has the outstanding advantages of specificity, sensitivity, high yield, rapidity, simplicity, good repeatability, and easy automation. It is widely used. In scientific research and various medical tests. The PCR tube is an essential consumable in the PCR reaction, providing a reaction place for the PCR reaction. PCR technology can amplify the target gene or a certain DNA fragment to be studied to 100,000 or even millions of times within a few hours in a PCR tube. During the reaction process, it is often necessary to observe the reaction degree in the tube and adjust the reaction conditions to achieve the purpose of controlling the reaction progress. [0003] In order to facilitate the operation of PCR experiment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L23/12C08L83/04C08K5/053
CPCC08L23/12C08L2203/18C08L2205/025C08L2205/03C08L83/04
Inventor 朱一明
Owner 海南科晶生物技术有限公司
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