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Abnormal karyotype image library and construction method for same

A chromosomal abnormality and construction method technology, applied in the directions of maps/plans/charts, instruments, educational tools, etc., can solve problems such as no feasible quality control substances

Active Publication Date: 2014-09-24
ATTACHED OBSTETRICS & GYNECOLOGY OSPITAL MEDICALCOLLEGE ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0008] In order to solve the problem that there is no feasible quality control substance in prenatal cytogenetic diagnosis, the inventor proposes the present invention

Method used

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  • Abnormal karyotype image library and construction method for same
  • Abnormal karyotype image library and construction method for same

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Embodiment Construction

[0016] 1. Routine specimen collection and culture: routinely take fetal amniotic fluid, villi tissue and other specimens for routine chromosomal disease laboratory diagnosis due to clinical diagnosis and treatment or prenatal diagnosis, process the specimens according to routine, and inoculate cells in 25cm 2 In the cell culture flask, inoculate 2 to 3 flasks for each case, add 3ml of RPMI-1640 cell culture medium, at 37°C, 5% CO 2 In medium culture, amniotic fluid cells were cultured for about 7 days to change the medium, the supernatant liquid was poured into another culture bottle, and 3ml of fresh culture medium was added, and the culture was continued until the cell confluency reached about 85%; the growth of the villous tissue was observed under the microscope on the second day after inoculation If there are spindle cells growing, replace with fresh culture medium, and continue to culture until the cell confluency reaches about 85%.

[0017] 2. Routine cell harvesting an...

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Abstract

The invention relates to an abnormal karyotype image library used in the medical field and a construction method for the same. Remainder adherent growth living cells used for chromosome disease diagnosis due to a diagnosis need and already confirmed to be provided with complicated chromosome abnormality are taken, and subjected to repeated generation amplification, cells are corrected, lowly-permeated, fixed and stored at minus 20 DEG C, then one type or more types of cell suspensions are taken and mixed according to the needed ratio, thus only one abnormal karyotype contained in each part of the cell suspensions is converted to various abnormal karyotypes with different ratios and easy to carry out misdiagnose, the image library is prepared through chromosome preparation and image capture, the difficulty and complexity of differential diagnosis and chimera diagnosis are improved by the prepared images, and more chromosome abnormal combined types of karyotype images can be prepared as needed; specimens are easy to get and waste redundant cells are used for teaching, technical examination, external quality assessment and resource sharing for chromosome analysis, and a remarkable effect is acted on timely discovery for diagnosis quality problems and improvement for a diagnosis level.

Description

technical field [0001] The invention relates to an abnormal chromosome karyotype image library and its construction method, which is mainly used in the cytogenetics diagnostic laboratory in the medical field for teaching and demonstration of chromosome karyotype analysis, technical assessment, inter-laboratory quality assessment, resource sharing and storage. storage. Background technique [0002] Chromosomes are the genetic material in the nucleus. Humans have 23 pairs of chromosomes, 22 of which are autosomes, and 1 pair is the sex chromosome that determines the sex of men and women. Genes carrying genetic information on chromosomes, of which DNA accounts for more than 90%, and RNA content varies with cell cycle and growth, generally accounting for 1% to 10%. [0003] Chromosomal structural abnormalities, abnormal numbers, and mosaicism are clinically manifested as chromosomal diseases, which are common birth defects, such as congenital stupidity, primary amenorrhea, and ...

Claims

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Application Information

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IPC IPC(8): G09B29/00
Inventor 翁炳焕
Owner ATTACHED OBSTETRICS & GYNECOLOGY OSPITAL MEDICALCOLLEGE ZHEJIANG UNIV
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