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Method for Extracting Radix Ginseng Polysaccharide by Composite Enzyme Method

A technology of compound enzymatic method and compound enzymatic hydrolysis, applied in the field of extraction of yellow ginseng polysaccharide, can solve problems such as large organ damage

Active Publication Date: 2016-08-24
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these drugs take effect quickly, they cause great damage to organs such as liver and kidney.

Method used

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  • Method for Extracting Radix Ginseng Polysaccharide by Composite Enzyme Method
  • Method for Extracting Radix Ginseng Polysaccharide by Composite Enzyme Method
  • Method for Extracting Radix Ginseng Polysaccharide by Composite Enzyme Method

Examples

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Effect test

Embodiment 1

[0022] (1) Microwave extraction: Take the perennial yellow ginseng, dry it at 60°C for 12 hours, pulverize it with a pulverizer, and pass it through a 60-mesh sieve; add water according to the ratio of solid to liquid at 1:30g / mL, adjust the pH value to 5, and set the temperature at 60°C , microwave power is 200W, microwave extraction 10min;

[0023] (2) Double-enzyme extraction: adjust the stirring speed to 1200r / min, first hydrolyze with 1.5% of yellow ginseng mass cellulase (50,000 U / g) for 4 hours, and then use 0.3% yellow ginseng mass papain (650,000 U / g) / g) Hydrolyze for 4 hours, inactivate the enzyme at 90°C for 10 minutes, and cool to room temperature to obtain the extract;

[0024] (3) Concentration and precipitation: Centrifuge the extract at 4500 r / min for 10 minutes, take the supernatant, and filter twice with double-layer filter paper, at a pressure of 0.07Mpa, concentrate under reduced pressure at 60°C until the volume is 1% of the volume of the extract. / 5, ad...

Embodiment 2

[0028] (1) Microwave extraction: Take the perennial yellow ginseng, dry it at 60°C for 12 hours, pulverize it with a pulverizer, and pass it through a 60-mesh sieve; add water according to the ratio of solid to liquid at 1:34g / mL, adjust the pH value to 5.5, and set the temperature at 55°C , microwave power is 220W, microwave extraction 8min;

[0029] (2) Double-enzyme extraction: adjust the stirring speed to 800r / min, first hydrolyze with 1.5% of yellow ginseng mass cellulase (50,000 U / g) for 3.5 hours, and then use 0.3% yellow ginseng mass papain (650,000 U / g) was hydrolyzed for 3.5 hours, the enzyme was inactivated at 92°C for 12 minutes, and cooled to room temperature to obtain the extract;

[0030] (3) Concentration and precipitation: Centrifuge the extract at 4500 r / min for 12 minutes, take the supernatant, and filter twice with double-layer filter paper under a pressure of 0.08Mpa. Concentrate under reduced pressure at 65°C until the volume is 1% of the volume of the ex...

Embodiment 3

[0034] (1) Microwave extraction: Take the perennial yellow ginseng, dry it at 60°C for 12 hours, pulverize it with a pulverizer, and pass it through a 60-mesh sieve; add water according to the ratio of solid to liquid at 1:38g / mL, adjust the pH value to 6.5, and set the temperature at 58°C , the microwave power is 250W, and the microwave extraction is 12min;

[0035] (2) Double-enzyme extraction: adjust the stirring speed to 1000r / min, first hydrolyze with 1.5% of yellow ginseng mass cellulase (50,000 U / g) for 3 hours, and then use 0.3% yellow ginseng mass papain (650,000 U / g) / g) Hydrolyze for 3 hours, inactivate the enzyme at 94°C for 15 minutes, and cool to room temperature to obtain the extract;

[0036] (3) Concentration and precipitation: Centrifuge the extract at 4000r / min for 15min, take the supernatant, and filter twice with double-layer filter paper, at a pressure of 0.09Mpa, concentrate under reduced pressure at 70°C until the volume is 1 / of the volume of the extra...

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Abstract

The invention discloses a process for extracting polysaccharides from yellow ginseng by compound enzyme method. The yellow ginseng is dried and crushed, then water is added, extracted by microwave, then hydrolyzed by cellulase, then hydrolyzed by papain, enzyme is inactivated, cooled, centrifuged, The supernatant was taken, pumped through double-layer filter paper, concentrated under reduced pressure, finally precipitated with ethanol, and freeze-dried to obtain polysaccharides from yellow ginseng. The yield of polysaccharides was 18.92-21.35%. The polysaccharide content in the extract was determined by the phenol-sulfuric acid method to be 88.33%±0.93%. The protein content was determined at 2.17±1.03% by Coomassie brilliant blue method. Experiments show that the yellow ginseng polysaccharide of the present invention has obvious inhibitory effect on blood sugar and blood lipid in experimental hyperglycemic rats, and is safe and has no side effects. The freeze-dried extract is crushed and made into tablets, hard capsules, granules, etc. It is a natural health care product with the function of regulating blood sugar and blood lipids.

Description

technical field [0001] The invention relates to a method for extracting yellow ginseng polysaccharides, in particular to a method for extracting yellow ginseng polysaccharides by a compound enzyme method. Background technique [0002] With the acceleration of the pace of life in today's society and the increase in life pressure, the number of people suffering from hypertension, hyperglycemia, and hyperlipidemia (also known as the three highs) is increasing day by day. Nowadays, the prevention and treatment of the three highs mainly depends on western medicine, such as Captopril Tablets, Compound Jiangya Tablets, Qingnao Jiangya Tablets, etc. for the prevention and treatment of high blood pressure. Drugs for hyperlipidemia such as simvastatin, Pragut, etc. Although these medicines take effect quickly, they cause greater damage to organs such as the liver and kidneys. The pure natural plant extracts have the advantages of mild action, no toxic side effects, etc., and have gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 张继宋珅程昉殷振雄刘琴赵保堂丁昊安珂付亚娟
Owner NORTHWEST NORMAL UNIVERSITY
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