Faeces sample stabilizing solution, preparation method and application

A technology for stabilizing liquids and samples, applied in the field of molecular biology, can solve the problems of inability to preserve, increase the workload of medical staff, and unstable samples, etc., and achieve the effect of facilitating detection and treatment, convenience and flexibility

Active Publication Date: 2014-10-01
HANGZHOU NEW HORIZON HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, for stool tests in hospitals or physical examination centers, samples cannot be preserved due to instability, and must be sent for testing immediately
This has caused some inconvenience to patients, increased workload for medical staff, and...

Method used

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  • Faeces sample stabilizing solution, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Prepare stool sample stabilization solution, the formula is as follows:

[0015] 0.02-4M Guanidine thiocyanate, 2-20mM Tris-HCl (pH=5-10), 0.05-0.1mM NaCI, 1-15mM EDTA.

[0016] Its preparation method is as follows:

[0017] Dissolve Tris in water and adjust the pH to 5-10 with hydrochloric acid to obtain Tris-HCl solution; dissolve ethylenediaminetetraacetic acid in water and adjust the pH to 5-10 with hydrochloric acid to obtain EDTA solution; Dissolve in water, add guanidine thiocyanate, Tris-HCl solution, and EDTA solution to mix, and then sterilize to obtain a stool sample stable solution.

[0018] Take the prepared stool sample stabilization solution, add the stool sample with the same volume as the stabilization solution, mix well; control the temperature within 50 degrees Celsius, and store.

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Abstract

The invention relates to the field of molecular biology, and discloses a faeces sample stabilizing solution. The stabilizing solution comprises the following components: 0.02M-4M Guanidine thiocyanate, Tris-HCI of which the pH is 5-10 and the concentration is 2-20 M, 0.05-0.1 mM NaCl and 1-15 mM EDTA. According to the invention, the stability of the detected components in the faeces sample can be effectively kept, self-help fetch by a patient at home can be possible, the faeces stabilizing solution cannot be a hindrance to downstream detection (nucleic acid extraction and detection), the mixture of the faeces and the stabilizing solution can directly enter a detection process, greatly facilitates the flexibility of the follow-up treatment process of the faeces sample, and is beneficial to the detection and the treatment of digestive system diseases.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a stool sample stabilization solution. Background technique [0002] At present, for stool tests in hospitals or physical examination centers, the samples cannot be preserved due to instability, and must be sent for examination immediately. This has caused certain inconvenience to patients, increased the workload of medical staff, and also increased the difficulty of operating and providing results in a timely manner for testing laboratories. Therefore, the proportion of patients who cooperate in providing samples decreases, which affects the effectiveness of subsequent related medical methods. Contents of the invention [0003] The purpose of the present invention is to provide a stool sample stabilization solution that can effectively maintain the stability of the detected components in the stool sample, is non-toxic or corrosive, does not hinder downstream detection, and is...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/10
CPCC12Q1/6806C12Q2523/113
Inventor 岳汉军陈一友吕宁
Owner HANGZHOU NEW HORIZON HEALTH TECH CO LTD
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