Penicillin fermentation process fault diagnosis method based on kernel partial least squares reconstitution

Abstract

The invention provides a penicillin fermentation process fault diagnosis method based on kernel partial least squares reconstitution. The method comprises the following steps that: off-line historical normal data in the penicillin fermentation process is collected; a penicillin fermentation process operating variable off-line historical normal data set and a penicillin fermentation process state variable off-line historical normal data set are respectively normalized and standardized; an improved kernel partial least squares method is used for building a fault monitoring model of the penicillin fermentation process; faults in the penicillin fermentation process are monitored on line; a penicillin fermentation process fault correlation direction model based on the improved kernel partial least squares reconstitution is built; and the penicillin fermentation process fault diagnosis is carried out. According to the method provided by the invention, an input space is divided into a principal element space directly relevant to the output, a principal element space irrelevant to the output and a residual error space irrelevant to the output. Compared with a traditional method, the penicillin fermentation process fault diagnosis method has the advantages that input variables relevant to the output are monitored, and variables relevant to the input are also precisely monitored.

Description

technical field [0001] The invention belongs to the technical field of fault monitoring and diagnosis, and in particular relates to a method for fault diagnosis of penicillin fermentation process based on nuclear partial least squares reconstruction. Background technique [0002] Penicillin is a kind of antibiotic and a secondary metabolite. The schematic diagram of its fermentation process is shown in figure 1 As shown, the entire production cycle includes 4 physiological periods: the reaction lag period, the rapid growth period of the bacteria, the penicillin synthesis period and the death (autolysis) period of the bacteria; 2 physical sub-periods: the cell culture period (also known as the batch operation stage, corresponding to the first two physiological periods, lasting about 45h) and the penicillin fed-feeding fermentation stage (also known as the intermittent feeding operation stage, corresponding to the last two physiological periods, lasting about 355h). During th...

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Problems solved by technology

When controlling foam, it is also necessary to add acid and alkali to adjust the pH value of fermentation. Too much or too little acid and alkali will affect the pH value of fermentation, resulting in the failure of penicillin fermentation

In addition, in the cell culture stage, the concentration of bacteria is low, and the medium is rich in nutrients, so it is easy to form bacterial contamination.

In the early stage of penicillin fermentation, miscellaneous bacteria are easy to reproduce after infection, and compete with the production bacteria for nutrients and oxygen, which seriously interferes with the growth and reproduction of production bacteria and the production of products, disturbs the production order, and disrupts the production plan; the contamination in the middle stage of fermentation will seriously interfere with production The metabolism of bacteria affects the production of products. At this time, the method of "inverting" is generally adopted, which will cause a lot of waste of raw materials and increase of operating costs.

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