Artificial breeding method for acipenser dabryanus

Abstract

The invention relates to the technical field of aquaculture, in particular to an artificial breeding method for acipenser dabryanus. Oxytocin is injected into female acipenser dabryanus two times, wherein the interval between two times of injection is 12 hours, and the injection amount of the first time accounts for 1 / 3 to 1 / 2 of the total dose. The one-time injection method is adopted for male acipenser dabryanus, wherein injection of the male acipenser dabryanus is synchronous with the first time of injection of the female acipenser dabryanus, and the dose of the male acipenser dabryanus is half that of the female acipenser dabryanus. Fertilization is conducted according to a half-dry method, water, eggs and seminal fluid are mixed according to the proportion of 75:50:1, and the mixture is stirred for 2 minutes to 3 minutes. A complete acipenser dabryanus artificial breeding technique process is provided, and technical support is provided for large-scale artificial breeding; due to the adoption of the method, the hatchability of acipenser dabryanus can reach 85% or higher at most, and restrict factors in the acipenser dabryanus artificial breeding process are eliminated.

Description

technical field

[0001] The invention relates to the technical field of aquaculture, in particular to an artificial propagation method of Dabry's sturgeon. Background technique

[0002] Dabry's sturgeon is a unique flagship species and an important economic fish in the Yangtze River Basin of my country. Its breeding and wild population expansion are related to the development of my country's characteristic aquaculture, the health of the aquatic ecosystem and the safety of important and unique aquatic germplasm resources. However, due to the severe disturbance of the water ecosystem in the basin by human activities (such as hydropower development, water pollution and illegal fishing, etc.), the population of Acipenser dabryi has been reduced, and even endangered. In response to the severe situation of its population change, we have carried out a series of ecological and reproductive research, mastered certain key ecological data about its reproduction and feeding habits, and r...

Examples

Embodiment 1

[0012] 1. Under natural conditions, when the water temperature reaches 16°C, start to pay attention to the activities of the Dabry's sturgeon in the pond, and observe the eggs by perforating the abdomen. Indicators to judge the breeding timing of Acipenser dabryi.

[0013] 2. Before artificial breeding, they were kept in a micro-flow pool for 7 days, and the female fish were injected with LHR-A26ug + carp pituitary gland 1.5 capsules / kg of oxytocic agent, using the second injection method with an interval of 12 hours. The first injection was 1 of the total dose. / 3; The male fish is injected with one injection, the time is synchronized with the first injection of the female fish, and the dose is half of the female fish; after the injection, both the female fish and the male fish are temporarily kept in the micro-flow pond. (LHR-A2 is luteinizing hormone releasing hormone A2 produced by Ningbo Sansheng Pharmaceutical Co., Ltd.).

[0014] 3. 20 hours after the first injection, ...

Embodiment 2

[0024] 1. Under natural conditions, when the water temperature reaches 16°C, start to pay attention to the activities of the Dabry's sturgeon in the pond, and observe the eggs by perforating the abdomen. Indicators to judge the breeding timing of Acipenser dabryi.

[0025] 2. Before artificial breeding, they were kept in a micro-flow pool for 9 days, and the female fish were injected with LHR-A26ug + carp pituitary 1.5 capsules / kg of oxytocic agent, using the second injection method with an interval of 12 hours. The first injection was 1 of the total dose. / 2; The male fish is injected with one injection, the time is synchronized with the first injection of the female fish, and the dose is half of the female fish; after the injection, both the female fish and the male fish are temporarily kept in the micro-flow pond. (LHR-A2 is luteinizing hormone releasing hormone A2 produced by Ningbo Sansheng Pharmaceutical Co., Ltd.).

[0026] 3. 20 hours after the first injection, start ...

technical field

[0001] The invention relates to the technical field of aquaculture, in particular to an artificial propagation method of Dabry's sturgeon. Background technique

[0002] Dabry's sturgeon is a unique flagship species and an important economic fish in the Yangtze River Basin of my country. Its breeding and wild population expansion are related to the development of my country's characteristic aquaculture, the health of the aquatic ecosystem and the safety of important and unique aquatic germplasm resources. However, due to the severe disturbance of the water ecosystem in the basin by human activities (such as hydropower development, water pollution and illegal fishing, etc.), the population of Acipenser dabryi has been reduced, and even endangered. In response to the severe situation of its population change, we have carried out a series of ecological and reproductive research, mastered certain key ecological data about its reproduction and feeding habits, and r...

Examples

Embodiment 1

[0012] 1. Under natural conditions, when the water temperature reaches 16°C, start to pay attention to the activities of the Dabry's sturgeon in the pond, and observe the eggs by perforating the abdomen. Indicators to judge the breeding timing of Acipenser dabryi.

[0013] 2. Before artificial breeding, they were kept in a micro-flow pool for 7 days, and the female fish were injected with LHR-A26ug + carp pituitary gland 1.5 capsules / kg of oxytocic agent, using the second injection method with an interval of 12 hours. The first injection was 1 of the total dose. / 3; The male fish is injected with one injection, the time is synchronized with the first injection of the female fish, and the dose is half of the female fish; after the injection, both the female fish and the male fish are temporarily kept in the micro-flow pond. (LHR-A2 is luteinizing hormone releasing hormone A2 produced by Ningbo Sansheng Pharmaceutical Co., Ltd.).

[0014] 3. 20 hours after the first injection, ...

Embodiment 2

[0024] 1. Under natural conditions, when the water temperature reaches 16°C, start to pay attention to the activities of the Dabry's sturgeon in the pond, and observe the eggs by perforating the abdomen. Indicators to judge the breeding timing of Acipenser dabryi.

[0025] 2. Before artificial breeding, they were kept in a micro-flow pool for 9 days, and the female fish were injected with LHR-A26ug + carp pituitary 1.5 capsules / kg of oxytocic agent, using the second injection method with an interval of 12 hours. The first injection was 1 of the total dose. / 2; The male fish is injected with one injection, the time is synchronized with the first injection of the female fish, and the dose is half of the female fish; after the injection, both the female fish and the male fish are temporarily kept in the micro-flow pond. (LHR-A2 is luteinizing hormone releasing hormone A2 produced by Ningbo Sansheng Pharmaceutical Co., Ltd.).

[0026] 3. 20 hours after the first injection, start ...