Serratia liquefaciens A13 as well as fermentation culture method and application thereof

A technology of Serratia and fermentation culture, applied in the field of microbial strains, can solve the problems of microbial ecological imbalance, affecting the sustainable development of aquaculture industry, increasing bacterial resistance, etc.

Active Publication Date: 2014-12-10
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, traditional methods such as chemically synthesized drugs and antibiotics are mostly used in the prevention and control of loach disease, which not only increases bacterial drug resistance, causes microbial ecological imbalance,

Method used

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  • Serratia liquefaciens A13 as well as fermentation culture method and application thereof
  • Serratia liquefaciens A13 as well as fermentation culture method and application thereof
  • Serratia liquefaciens A13 as well as fermentation culture method and application thereof

Examples

Experimental program
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Embodiment 1

[0053] Embodiment 1, a kind of Serratia liquefaction ( Serratia liquefaciens ) A13, its deposit number is: CGMCC No.9490.

Embodiment 2

[0054] Embodiment 2, a kind of fermentative cultivation method of Serratia liquefaction A13 as described in embodiment 1, its fermentation condition is: select YPG or NYBD as substratum for use, liquid filling volume is 125 mL / 500mL Erlenmeyer flask, substratum The initial pH is 7.0, the culture temperature is 40°C, and the harvest time is 22-26 h; the composition of the medium YPG is: peptone 5 g / L, glucose 5 g, yeast extract 5 g, and distilled water 1 L; The composition of medium NYBD was: glucose 10 g, beef extract 8 g, yeast extract 5 g, and distilled water 1 L.

[0055] Serratia liquefaction A13 described in embodiment 1 or the bacteriostasis that the method described in embodiment 2 cultivates the bacteriostasis that obtains can be used to suppress Aeromonas vanrona ( Aeromonas veronii ), can be used for the preparation of loach breeding medicine, or be used for adding in the loach breeding feed as antibacterial medicine.

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Abstract

The invention discloses Serratia liquefaciens A13 with a preservation number: CGMCC No.9490. The invention discloses a fermentation culture method for the Serratia liquefaciens A13. The fermentation culture method comprises the following steps: selecting YPG or NYBD as a culture medium, and a triangular flask with liquid filling amount of 125mL/500mL, wherein an initial pH value of the culture medium is 7.0, a culture temperature is 40 DEG C and harvest time is 22-26 hours. The Serratia liquefaciens A13 or bacterial liquid obtained by culture by virtue of the method has a bacteriostatic purpose of inhibiting Aeromonas veronii, can be used for preparing a misgurnus anguillicaudatus breeding drug or can be used as a bacteriostatic drug to be add into misgurnus anguillicaudatus breeding feed.

Description

technical field [0001] The invention relates to a microbial strain, in particular to a Serratia liquefaction A13; the invention also relates to a fermentation culture method and application of the bacteria. Background technique [0002] Loach ( Misgurnus anguillicaudatus ), is favored by people for its excellent nutrient composition structure, and its aquaculture industry has developed rapidly in recent years. However, while bringing delicious food to people and bringing economic benefits to fishermen, its disease problem has become increasingly prominent. Bacterial diseases of loach are mainly caused by infections such as Aeromonas temperatus, Vibrio vulnificus, Aeromonas loach and Aeromonas vanlon. It is distributed all over the place. At present, traditional methods such as chemically synthesized drugs and antibiotics are mostly used in the prevention and control of loach disease, which not only increases bacterial drug resistance, causes microbial ecological imbalanc...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/18A23K1/17C12R1/425A23K20/195
Inventor 安贤惠李联泰赵艳景张生武
Owner HUAIHAI INST OF TECH
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