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A kind of compound stabilizer used in α-hydroxybutyrate dehydrogenase assay kit

A technology of hydroxybutyrate dehydrogenase and composite stabilizer, which is applied in the direction of measuring device, color/spectral characteristic measurement, instrument, etc., can solve the problem of high cost, complicated method, and cannot guarantee that the production rate and total amount of NADH can meet the requirements of the kit. requirements, etc., to achieve the effect of high accuracy, simple preparation, and prolonging the validity period of the bottle.

Active Publication Date: 2017-04-19
SUZHOU COMING CHENGYE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Patent CN02139298 mentions the addition of oxidized nicotinamide coenzyme (NAD + or NADP + ), using the enzyme-substrate-NAD + or enzyme-substrate-NADP + The system circulation converts the oxidized coenzyme into the reduced coenzyme, which can be measured when the reduced coenzyme accumulates to a certain concentration, but this method is complicated, expensive, and cannot guarantee the production rate and total amount of NADH in the system circulation. Meet the requirements of the kit; patent CN200610030784.8 provides a composite stabilizer for NADH, which has a certain stabilizing effect on NADH alone, but it does not mention that it can also play a similar role when added to a kit with more complex components. stabilizing effect

Method used

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  • A kind of compound stabilizer used in α-hydroxybutyrate dehydrogenase assay kit
  • A kind of compound stabilizer used in α-hydroxybutyrate dehydrogenase assay kit
  • A kind of compound stabilizer used in α-hydroxybutyrate dehydrogenase assay kit

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Experimental program
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Embodiment 1

[0018] Provide a kind of composite stabilizer for α-hydroxybutyrate dehydrogenase assay kit, the content of described composite stabilizer component is: Trishydroxymethylaminomethane 6 g / L, disodium edetate 50 g / L, trehalose 25 g / L, bovine serum albumin 20 g / L, glycerol 15 g / L, polyvinylpyrrolidone 20 g / L, Proclin 300 5 g / L, fully dissolve the above components, After mixing, a finished liquid composite stabilizer can be obtained.

[0019] Example 1 was applied to the α-hydroxybutyrate dehydrogenase assay kit, and evaluated from three aspects of accuracy, stability and usage.

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Abstract

The invention discloses a composite stabilizer for an alpha-hydroxybutyric dehydrogenase assay kit. The composite stabilizer contains a variety of ingredients, such as a buffer solution, protein, a chelating agent, polyol and a preservative, and contains the ingredients by content: 3-9g / L of trihydroxymethyl aminomethane, 40-60g / L of disodium edetate, 20-30g / L of trehalose, 10-30g / L of bovine serum albumin, 10-20g / L of glycerine, 15-25g / L of polyvinyl pyrrolidone and 1-10g / L of Proclin300. Through the manner, by applying the composite stabilizer disclosed by the invention to the alpha-hydroxybutyric dehydrogenase assay kit, the accuracy of assay results is high, the stability is good, and the bottle opened effective period is prolonged. In addition, the composite stabilizer is low in cost and simple in preparation, thereby being worthy of further popularization.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a composite stabilizer used in an alpha-hydroxybutyrate dehydrogenase assay kit. Background technique [0002] α-Hydroxybutyrate dehydrogenase (HBDH) is not an independent specific enzyme, but a general term for lactate dehydrogenase isozymes LDH1 and LDH2 containing H subunits. Determination of α-hydroxybutyrate dehydrogenase actually reflects the activity of lactate dehydrogenase isoenzymes LDH1 and LDH2, which is of great significance for the diagnosis of myocardial disease and liver disease. At present, the α-hydroxybutyrate dehydrogenase kits used in many domestic medical institutions are mostly determined by the rate method. The principle of the test is as follows: [0003] . [0004] α-hydroxybutyrate dehydrogenase catalyzes the conversion of α-ketobutyrate to α-hydroxybutyrate and simultaneously oxidizes nicotinamide adenine dinucleotide (NADH) to NAD + , the rate ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33G01N33/573
Inventor 杨灵勇周丽霞
Owner SUZHOU COMING CHENGYE MEDICAL TECH