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Composite stabilizer for aspartate aminotransferase assay kit

An aspartic acid amino, compound stabilizer technology, applied in the direction of color/spectral property measurement, etc., can solve the problem that the detection kit cannot be applied in a neutral environment, the effective stability period is not specified, and the cost of liposome manufacturing is high. problem, to achieve the effect of prolonging the opening period, excellent stability and low cost

Inactive Publication Date: 2015-03-04
SUZHOU COMING CHENGYE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent CN87100939 provides a NADH stabilizing composition, which includes propylene glycol, boric acid and alkaline buffer. Although this composition can maintain the stability of NADH alone, it cannot be applied to the aspartic acid amino group in a neutral environment. In the transferase detection kit; patent CN200510023147.3 provides a stabilizer for improving the rate method detection kit—dithiothreitol, but it is aimed at the storage time after adding the stabilizer to the unopened kit, and The effective stability period after opening is not specified; US Patent US5654288 discloses a method of encapsulating NADH with liposomes, but the manufacturing cost of liposomes is relatively high
It can be seen that the existing patents are all aimed at the stability of NADH alone, and do not mention aspartate aminotransferase assay kits with complex components and more enzyme types. A stabilizer with a good stabilizing effect is very necessary

Method used

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  • Composite stabilizer for aspartate aminotransferase assay kit
  • Composite stabilizer for aspartate aminotransferase assay kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Provide a kind of compound stabilizer that is used for aspartate aminotransferase assay kit, the content of each component of described compound stabilizer is: trishydroxymethylaminomethane 6.057 g / L, disodium edetate 25 g / L, trehalose 25 g / L, gelatin 20 g / L, propylene glycol 15 g / L, Brij-35 5 g / L, Proclin 300 1 g / L. After the above-mentioned components are fully dissolved and mixed, a finished liquid composite stabilizer can be prepared.

[0017] Example 1 was applied to the aspartate aminotransferase assay kit, and evaluated from the aspects of stability, accuracy and usage.

[0018] (1) Stability and accuracy test

[0019] 1. Stabilizer for testing: composite stabilizer described in Example 1.

[0020] 2. The test reagent is the Aspartate Aminotransferase Assay Kit, which consists of Reagent 1 and Reagent 2. The components are as follows:

[0021] Reagent 1: Tris 9.691 g / L, Sodium Hydroxide 0.935 g / L, Aspartic Acid 23.958 g / L, Nicotinamide Adenine Dinucleotide 0....

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Abstract

The invention discloses a composite stabilizer for an aspartate aminotransferase assay kit. The composite stabilizer comprises trihydroxymethyl aminomethane, disodium ethylenediaminetetraacetate, trehalose, gelatin, propylene glycol, a surfactant Brij-35 and a liquid biological preservative Proclin300. Through the technical scheme, the composite stabilizer for the aspartate aminotransferase assay kit comprises the components of a buffer solution, a stabilizer, a chelating agent, an organic solvent, a surfactant and a preservative and the like. After the composite stabilizer is added into the aspartate aminotransferase assay kit in a certain volume ratio, not only the accuracy of an assay result can be ensured, but also the stability thereof is higher, and the expiry date after the aspartate aminotransferase assay kit is opened can be effectively prolonged.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a composite stabilizer used in an aspartate aminotransferase assay kit. Background technique [0002] Aspartate aminotransferase (AST), formerly known as aspartate aminotransferase, widely exists in cardiac muscle, skeletal muscle, and liver, with the highest content in cardiac muscle, followed by liver. The determination of aspartate aminotransferase can reflect the degree of liver cell damage, and its increase is common in liver diseases such as infectious hepatitis, myocardial infarction, dermatomyositis, muscle injury, etc. It is a very common clinical biochemical test item for liver function , which is of great significance for clinical diagnosis. [0003] At present, the detection in serum mainly adopts the continuous monitoring method, and the specific determination process is as follows: [0004] . [0005] Aspartate and α-ketoglutarate are converted into oxaloaceta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
Inventor 周立霞杨灵勇王燕华李勋波
Owner SUZHOU COMING CHENGYE MEDICAL TECH
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