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Chlamydospore fusarium strain and application thereof

A technology of Fusarium and spores, which is applied to the strain of Fusarium pachysporum, the spores of Fusarium pachysporum ZC201301, and the application field of the Fusarium chlamysporum strain to achieve the effect of significant economic and social benefits

Active Publication Date: 2014-12-31
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Nanjing Agricultural University has applied for a patent for biological control of crabgrass crabgrass by using Nakataea Hara strain QZ-2000 (preservation number: CGMCC No.0640), but its single-strain control effect It is not ideal, and the spores of several individual plants need to be mixed in proportion and then sprayed to achieve better control effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Isolation and identification of Fusarium chlamydosporum strain ZC201301

[0033] 1.1 Isolation of strains

[0034] Collect naturally susceptible crabgrass leaves in the field, cut off the stalks and healthy joints of the diseased leaves, rinse them with sterile water for 3 times, and disinfect the surface with 2% (v / v) sodium hypochlorite solution for 6 minutes, and then use 70% ( v / v) Alcohol surface disinfection for 1 minute, after rinsing with sterile water, transfer these tissues to an empty sterilized petri dish, dry them in an ultra-clean bench, place them in an incubator at 28°C, and undergo single spore separation and purification The pure culture strain ZC201301 was obtained.

[0035] 1.2 Pathogenicity of ZC201301 strain to crabgrass

[0036] After the ZC201301 strain was cultured on PDA medium for 10 days, the spores were collected and prepared at a concentration of 1×10 7 Individual / mL spore suspension was sprayed on 2 leaves and 1 heart stage c...

Embodiment 2

[0046] Embodiment 2: the preparation of spore liquid

[0047] Inoculate Fusarium chlamydoides ZC201301 on PDA plate medium, and culture it for 12 days at 28°C under alternating light and dark conditions, and there are a large number of conidia produced by the strain on the surface of the medium. Add Tween 80 accounting for 0.1% of the total mass, gently brush off the spores growing on the surface of the medium with a cotton swab, collect the spore liquid, count with a hemocytometer under a microscope and adjust the concentration of the spore liquid to 1×10 7 individual / mL. The prepared spore suspension is directly used for biological control of weeds such as crabgrass.

Embodiment 3

[0048] Embodiment 3: Determination of the host range of bacterial strain ZC201301 CGMCC

[0049]It is clear from the determination of the host range that the strain ZC201301 is safe to soybean, corn, wheat and other crops; %about.

[0050] Table 1 Determination of the host range of strain ZC201301

[0051] Calculation formula of fresh weight inhibition rate: fresh weight inhibition rate = (control average fresh weight - treatment average fresh weight) / control average fresh weight × 100%.

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PUM

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Abstract

The invention relates to the technical field of fusarium strains, and in particular relates to a chlamydospore fusarium strain ZC201301. The preservation number of the chlamydospore fusarium strain is CGMCC No.9562. The chlamydospore fusarium strain is separated from leaves of natural pathogenetic digitaria sanguinalis in the field and is strong and quick in pathogenicity to the digitaria sanguinalis; an indoor experiment shows that spores of the strain disclosed by the invention are sprayed to the leaves of the digitaria sanguinalis; after two days, spots appear on the digitaria sanguinalis, and part of the digitaria sanguinalis is withered; after 14 days, the fresh weight suppression rate to the digitaria sanguinalis is 91 percent. The chlamydospore fusarium strain ZC201301 which is a fungus strain has a certain biological prevention effect on grasses such as amaranthus retroflexu and fagopyrin and is safe to crops such as soybeans, corns and wheat.

Description

[0001] technical field [0002] The invention relates to the technical field of Fusarium chlamydoides, in particular to a Fusarium chlamydoides strain, and also relates to the application of the Fusarium chlamydoides strain. [0003] Background technique [0004] Crabgrass (Diditaria sanguinaris) is one of the most widely distributed malignant weeds in the world. It mainly harms wheat, barley, corn, rice, sorghum and other gramineous crops, as well as vegetable gardens and lawns. In China, the damaged area of ​​crabgrass crabgrass is about 60 million hectares, and the crop loss caused by it accounts for more than half of the total loss of weeds in autumn dry crop fields. Higher frequency. At present, Nanjing Agricultural University has applied for the use of Bifucus ( Nakataea Hara ) strain QZ-2000 (preservation number: CGMCC No.0640) is a patent for biological control of crabgrass, but its single-strain control effect is not ideal, and the spores of several individual pl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/04A01P13/00C12R1/77
Inventor 李健李美高兴祥房锋
Owner INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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