Curvularia lunata strain and application thereof

A technology of Curvularia and Crescent, which is applied in the field of Curvularia crescentus strains, can solve the problems of failure to develop biological barnyard-control agents, and achieve the effects of low development cost, reduced production cost, and rapid pathogenicity

Inactive Publication Date: 2010-08-25
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far commercialized biological barnyard

Method used

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  • Curvularia lunata strain and application thereof
  • Curvularia lunata strain and application thereof

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0034] Example 1. Isolation and identification of strains

[0035] 1. Separation of strains

[0036] (1) Collect naturally susceptible barnyard grass leaves in the wild. (2) Remove several pieces of tissue about 4 mm in length from the diseased and healthy joints of the diseased leaf, and disinfect the surface with 2% (v / v) sodium hypochlorite solution for 10 minutes, repeat 3 times; then use 65% (v / v) v) Alcohol surface disinfection for 1 minute, repeat 3 times. Finally, rinse with sterile water. Transfer these tissues to PDA plates and place them in a 28°C incubator for cultivation. (3) Pure cultured bacteria are obtained after monospore separation and purification.

[0037] 2. Screening of strains

[0038] Leaf segment method: Cut off the third leaf of barnyardgrass, cut out a 4cm-long leaf segment with a flat middle, and stick it on the surface of a water agar (containing benzimidazole 0.05 / L) flat plate face up. Wash the spores of the tested strain with sterile water and adj...

Example Embodiment

[0050] Example 2: Obtaining of spores and preparation of spray

[0051] 1. Acquisition, collection and storage of spores

[0052] (1) Method I

[0053] Inoculate Curvularia lunata B6 CGMCC No. 2928 on a PDA plate medium, culture for 14 days at 28°C and dark conditions, the surface of the medium is the conidia produced by the strain; brush it with a brush The spores grown on the surface of the culture medium are collected and the spore powder is directly used to make barnyardgrass biological control agents, or stored in a refrigerator at 4°C, and taken out when needed.

[0054] The composition of PDA medium: 20g glucose, 15g agar, 200g potato extract, distilled water to make the volume to 1L. The 200g potato extract is prepared according to the following method: Weigh 200g potato, wash, peel and cut into small pieces, add 1000ml of water and boil for half an hour, filter with gauze, and take the filtrate to obtain 200g potato extract.

[0055] Results: The yield of spores can reach at ...

Example Embodiment

[0071] Example 3. Application of biological agents

[0072] 1. Control of barnyardgrass by biological agents

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Abstract

The invention provides a curvularia lunata strain and application thereof. The strain is curvularia lunata B6 of which the preservation number is CGMCC No.2928. The curvularia lunata strain B6 CGMCC No.2928 has strong and fast pathogenicity to echinochloa crusgalli; field experiments show that the echinochloa crusgalli shows spot and dead symptoms in a week after spores of the curvularia lunata strain are sprinkled to the echinochloa crusgalli and the fresh weight inhibition rate to the echinochloa crusgalli can reach 90 percent in 28 days. The fungus strain curvularia lunata strain B6 CGMCC No.2928 is separated from echinochloa crusgallis naturally having diseases in fields without any artificial gene modification; therefore, the curvularia lunata strain cannot have any ecological and environmental hidden danger when released to nature and is an absolutely-safe biological prevention and control medium.

Description

technical field [0001] The invention relates to a strain of Curvularia lunata strain and application thereof. Background technique [0002] Barnyardgrass (Echinoloa crusgalli (L.) Beauv), belonging to the family Gramineae, is distributed in tropical and temperate regions and is ubiquitous throughout the country. It is one of the most serious weed species harmful to crops. Barnyardgrass is one of the vicious weeds in rice fields, which is associated with rice and is extremely difficult to remove. It also occurs in wet early land and harms autumn dry land crops such as cotton and soybean. Typically, as weed density increases, its competitiveness increases and crop yield losses increase. C as a high photosynthetic rate 4 plants, in CO 2 When the concentration is low, barnyardgrass on CO 2 The utilization rate is greater than that of C 3 Plants of rice and soybeans. Strong adaptability, like warm and humid environment, not only can grow in shallow water but also more drou...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00A01N63/04A01P13/02C12R1/645
Inventor 倪汉文李静韦韬曹永松王琦
Owner CHINA AGRI UNIV
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