Making method of immune complex adsorption cells

Abstract

The invention discloses a making method of immune complex adsorption cells. The method comprises the steps of vector construction, virus packaging production, immortalized B lymphocyte production and gene transfection. A set of immortalized cells respectively carrying specific genes is made, has immortalization (infinite proliferation) ability, can express a certain immune complex receptor on the surfaces on the cells, and an immune complex analysis group is established through amplifying cultured cells, and allows all immune complexes in blood or body tissues to be identified, classified and quantified. The method has the characteristics of low cost, infinite proliferation, fast detection speed, simple operation and the like. The aim of the invention is to obtain the immortalized B lymphocytes, the B lymphocytes can stably express an immune complex receptor gene, the receptor can exist on cell membranes, and can be combined with one corresponding immune complex not limited to immortalized B cells, outside the cells,.

Description

technical field [0001] The invention relates to a method for making cells, in particular to a method for making immune complexes to adsorb cells. Background technique [0002] Immune complex: a complex obtained by the combination of an antibody and an antigen is called an immune complex, which is formed by the combination of various immune cells that phagocytize bacteria, viruses, and allergenic substances and die together, so it is also called antigen-antibody Complex. Under normal circumstances, small molecular soluble immune complexes are filtered out by the glomerulus, and large molecular insoluble immune complexes are phagocytized and eliminated by macrophages, which is part of the body's defense mechanism. However, in some cases, the immune complexes formed by antigens and antibodies in the body are deposited at the base of the blood vessel wall, thereby activating complement, and the activated complement can dissolve bacteria, viruses, and tumor cells. [0003] Immo...

AI Technical Summary

Problems solved by technology

This method has high sensitivity and is easy to carry out in general laboratories, but the disadvantage is poor specificity

The third piece of evidence is sometimes difficult to find, but at least the first two pieces must be met, and the determination of CIC alone is not enough evidence

[0050] There are various deficiencies in the existing technologies, and it is impossible to quickly and effectively separate various immune complexes. The existing cell adsorption and separation technologies mainly use existing immunoglobulins carrying multiple or single complements or immunoglobulins. Recipient cells, these extensions have limited function and can bind to a limited number of types

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