Sparassis crispa strain separated culturing method

A technology for isolating and cultivating and drying bacteria, which is applied in the directions of botanical equipment and methods, cultivation, and plant cultivation, and can solve the problems of low survival rate, difficulty in artificial cultivation, and inability to ensure the stability of bacterial cultivation and reproduction.

Active Publication Date: 2015-03-04
罗星野 +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As the wild Dried Mushroom is a rare wild edible fungus unique to Yunnan, especially the Yunnan pine forest that grows in central Yunnan has the best taste. Since it belongs to ectophytic mycorrhizal fungi, it is difficult to achieve artificial cultivation, although there are currently some artificial cultivation method, all exist problems such as low survival rate, can not guarantee the stability of bacterial classification cultivation and reproduction, therefore, it is very necessary to develop a kind of method for the separation and cultivation of dry bacteria that can solve the above-mentioned technical problems

Method used

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  • Sparassis crispa strain separated culturing method
  • Sparassis crispa strain separated culturing method
  • Sparassis crispa strain separated culturing method

Examples

Experimental program
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Effect test

Embodiment 1

[0039] ——Preparation of culture medium for the isolation of D.

[0040]Medium formula: potato 150g, glucose 10g, agar 20g, peptone 5g, pine bark 40g, potassium dihydrogen phosphate 2g, magnesium sulfate 4g, the preparation method is as follows:

[0041] Wash and peel the potatoes, weigh them according to the proportion of the formula, cut them into 0.4cm pieces, boil them at 95°C for 4 minutes in water with a volume ratio of 1 times that of the reinforcement solution, and filter with double-layer gauze to obtain the filtrate a; weigh the pine trees according to the proportion of the formula Skin, water with twice the volume ratio of the reinforcement solution, boiled at 95°C for 4 minutes, filtered to obtain filtrate b; combined filtrate a and filtrate b, added agar powder, glucose, peptone, KH at 95°C 2 PO 4 and MgSO 4 , stirred and melted with a glass rod, transferred to a test tube or a petri dish, under a pressure of 1.5Kg / cm 2 , Sterilized at 121°C for 30-40 min...

Embodiment 2

[0043] ——Preparation of culture medium for the isolation of D.

[0044] Medium formula: potato 250g, glucose 30g, agar 30g, peptone 7g, pine bark 40g, pine hair 20g, potassium dihydrogen phosphate 4g, magnesium sulfate 6g, the preparation method is as follows:

[0045] Wash and peel the potatoes, weigh them according to the formula ratio, cut them into 0.6cm pieces, boil them in water with a volume ratio of 3 times the reinforcement solution at 110°C for 6 minutes, and filter them with double-layer gauze to obtain the filtrate a; weigh the pine bark according to the formula ratio and loose hair, water with a volume ratio of 5 times that of the reinforcing solution was boiled at 100°C for 10 minutes, filtered to obtain filtrate b; combined filtrate a and filtrate b, and added agar powder, glucose, peptone, KH at 110°C 2 PO 4 and MgSO 4 , stirred and melted with a glass rod, transferred to a test tube or a petri dish, under a pressure of 1.5Kg / cm 2 , and sterilized at 121°C f...

Embodiment 3

[0047] ——Preparation of culture medium for the isolation of D.

[0048] Medium formula: potato 200g, glucose 20g, agar 25g, peptone 6g, pine hair 50g, potassium dihydrogen phosphate 3g, magnesium sulfate 5g, the preparation method is as follows:

[0049] Wash and peel the potatoes, weigh them according to the proportion of the formula, cut them into 0.5cm slices, boil them at 100°C for 5 minutes with water whose volume ratio is 2 times that of the reinforcement solution, and filter with double-layer gauze to obtain the filtrate a; weigh the pine bark and / or loose hair, boil water with a volume ratio of 4 times that of the reinforcement solution at 98°C for 8 minutes, filter to obtain filtrate b; combine filtrate a and filtrate b, add agar powder, glucose, peptone, KH at 100°C 2 PO 4 and MgSO 4 , stirred and melted with a glass rod, transferred to a test tube or a petri dish, under a pressure of 1.5Kg / cm 2 , Sterilized at 124°C for 35 minutes.

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Abstract

The invention discloses a sparassis crispa strain separated culturing method. The method includes: sampling, namely sampling and storing puerile sparassis crispa fruit bodies selected from Yunnan pine land; performing surface sterilization, namely subjecting the collected sparassis crispa fruit bodies to surface sterilization; separating and purely culturing, namely cutting and disposing the sparassis crispa fruit bodies in a culture medium plane of a sparassis crispa tissue separating culture medium or a test tube bevel for culturing, inoculating into the culture medium plane of the sparassis crispa tissue separating culture medium or the test tube bevel, culturing to obtain a target object, and transferring the sparassis crispa fruit bodies into the test tube bevel of a sparassis crispa strain culture medium containing coniferous forest waste serving as a culture medium for storage. Living puerile fruit bodies of field sparassis crispa are selected and collected from the Yunnan pine land where sparassis crispa grows, tissue is quickly separated, and the coniferous forest waste like pine bark, pine chip and pine leaf is taken as the culture medium for culturing, so that a sparassis crispa strain is high in reproduction stability and high in growing vigor, and survival rate reaches more than 99.9%.

Description

technical field [0001] The invention belongs to the technical field of strain cultivation, and in particular relates to a method for separating and culturing D. Background technique [0002] Ganbajun (Thelephota.ganbajun.Zang) belongs to Basidiomycota, Phylomycetes, Phylomycetes, Aphyllomycetes, and is a large fungus of the genus Cortella. Wild dried fungus is a rare wild edible fungus unique to central Yunnan, and belongs to the ectomycorrhizal fungi that live in symbiosis with pine trees. According to scientific research records, dry dried mushrooms contain 24.15% crude protein, 8.45% crude fiber, 3.1% total sugar, 100g contains 1.10mg of vitamin C, 16.0mg of vitamin A, 1.2mg of B vitamins, and 45.14 mg of vitamin E; 18 kinds of amino acids and 8 kinds of amino acids necessary for the human body, the ratio of which is 55%, is a high-quality protein; various trace elements iron, potassium, sodium, phosphorus, zinc, magnesium, copper, manganese, calcium contained in dry bac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G3/00
CPCA01G18/00C05G5/40
Inventor 罗星野罗健陈天蓉
Owner 罗星野
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