Method for extracting DHA algae oil from algae cells

A technology of algae cells and algae oil, which is applied in the direction of fat oil/fat production, fat production, etc., can solve the problem of low extraction rate of DHA, achieve the effect of no organic solvent residue, improve extraction efficiency, and increase extraction rate

CN104388178AActive Publication Date: 2015-03-04SHANDONG BAILONG CHUANGYUAN BIO TECH

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Patent Information

Authority / Receiving Office: CN · China
Patent Type: Applications(China)
Current Assignee / Owner: SHANDONG BAILONG CHUANGYUAN BIO TECH
Publication Date: 2015-03-04

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Abstract

The invention relates to a method for extracting DHA algae oil from algae cells. The method comprises the following steps: (1) centrifuging and spray-drying algae cell fermentation broth to prepare algae cell dry powder, then uniformly mixing the algae cell dry powder with absolute ethanol, and carrying out high-pressure homogenizing cell wall breaking to prepare a wall-broken ethanol solution; (2) distilling the wall-broken ethanol solution at a low temperature in vacuum, removing ethanol to prepare wall-broken bacteria; and (3) carrying out CO2 supercritical extraction on the wall-broken bacteria to obtain DHA algae oil. According to the method, the algae cell dry powder is soaked by the absolute ethanol before high-pressure homogenizing cell wall breaking, so that the extraction rate can be greatly increased.

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Description

technical field

[0001] The invention relates to a method for extracting DHA algae oil from algae cells, belonging to the technical field of health product preparation. Background technique

[0002] Docosahexaenoic acid (DHA) is an important omega-3 long-chain polyunsaturated fatty acid. Modern medicine has proved that DHA can promote the visual and intellectual development of infants and young children, and also has a positive preventive effect on cardiovascular system diseases, cancer, and inflammation. The production of DHA has a variety of sources, among which the production of DHA algae oil by microbial fermentation has the advantages of easy large-scale cultivation, high polyunsaturated fatty acid content, easy separation and purification, and good oxidation stability. Among the many DHA-producing microorganisms (including Schizochytrium, Thraustochytrium, Cryptodinophyllum, etc.), Schizochytrium has many advantages such as fast growth and high DHA content, and is one ...

Claims

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