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A method for extracting DHA algae oil and algae protein from algae

A technology of algae and algae oil, which is applied in the preparation methods of peptides, chemical instruments and methods, fat oil/fat production, etc., can solve the problems of low extraction rate of DHA algal oil and its algal protein, and achieve mild conditions and improve the extraction rate. , to avoid damaging effects

Active Publication Date: 2017-12-01
SHANDONG BAILONG CHUANGYUAN BIO TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] However, the above-mentioned extraction process is limited by the technical conditions of the existing wall-breaking process conditions, and the extraction rate of DHA algae oil and its algae protein is still low

Method used

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  • A method for extracting DHA algae oil and algae protein from algae

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Effect test

Embodiment 1

[0050] A method for extracting DHA algae oil and algae protein from algae, comprising the steps of:

[0051] (1) Centrifuge the algae fermentation liquid through a disc centrifuge, the centrifugation conditions are 3000rpm, 3m 3 / h, take the precipitate, and prepare the bacterial liquid; then add absolute ethanol to the bacterial liquid, the weight-to-volume ratio of the bacterial liquid to absolute ethanol is 1:1, unit kg / L, let stand for 30min, and then Under the condition of temperature and pressure of 90Mpa, high-pressure homogeneous wall breaking was carried out, and the wall was broken 3 times in a cycle to obtain the broken bacteria liquid;

[0052] The preparation steps of the algae fermentation liquid are as follows:

[0053] 1. Get the algae and inoculate it in the activation medium, activate and cultivate it for 24 hours at 30°C to obtain the activated strain;

[0054] II, inoculate 10% by weight of the activated strains prepared in step I in the fermentation medi...

Embodiment 2

[0065] A method for extracting DHA algae oil and algae protein from algae, comprising the steps of:

[0066] (1) Centrifuge the algae fermentation liquid through a disc centrifuge, the centrifugation conditions are 3000rpm, 3m 3 / h, take the precipitate, and prepare the bacterial liquid; then add absolute ethanol to the bacterial liquid, the weight-to-volume ratio of the bacterial liquid and absolute ethanol is 0.5:1, the unit is kg / L, let stand for 30min, and then in 20 Under the temperature of ℃ and the pressure of 80Mpa, high-pressure homogeneous wall breaking was carried out, and the wall was broken 4 times in a cycle to obtain the broken bacteria liquid;

[0067] The preparation steps of the algae fermentation liquid are as follows:

[0068] 1. Get algae and inoculate them in the activation medium, and activate and cultivate them for 28 hours at 28°C to obtain activated strains;

[0069] II, inoculate the activated bacterial classification that step I makes by 5% by wei...

Embodiment 3

[0080] A method for extracting DHA algae oil and algae protein from algae, comprising the steps of:

[0081] (1) Centrifuge the algae fermentation liquid through a disc centrifuge, the centrifugation conditions are 3000rpm, 3m 3 / h, take the precipitate, and prepare the bacterial liquid; then add absolute ethanol to the bacterial liquid, the weight-to-volume ratio of the bacterial liquid to absolute ethanol is 2:1, the unit is kg / L, let stand for 30min, and then Under the condition of temperature and pressure of 120Mpa, high-pressure homogeneous wall breaking is carried out, and the wall breaking is circulated twice to obtain the broken bacteria liquid;

[0082] The preparation steps of the algae fermentation liquid are as follows:

[0083] 1. Get the algae and inoculate them in the activation medium, and activate and cultivate them for 20 hours at 32°C to obtain the activated strains;

[0084] II, inoculate the activated bacterial classification obtained in step I in the fe...

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Abstract

The invention relates to a method for extracting DHA algae oil and algae protein from algae. The method comprises the following steps: (1) centrifuging algae fermentation liquor, preparing a bacterium liquid from sediments, adding absolute ethyl alcohol to the bacterium liquid, and carrying out high-pressure homogeneous wall-breaking to prepare a broken bacterium liquid; (2) carrying out multi-stage extraction on the broken bacterium liquid through an organic solvent, so as to prepare extract liquor and mushroom dregs; (3) preparing DHA coarse algal oil on the extract liquor after low-temperature vacuum concentration, and carrying out alkali refining, degumming, and decoloring, so as to prepare DHA algal oil; and (4) centrifuging, washing and drying the mushroom dregs to prepare the algae protein. According to the method disclosed by the invention, algae are soaked by absolute ethyl alcohol before high-pressure homogenate wall-breaking, thus the extraction rate is greatly increased, and the extraction rate of the DHA algal oil can reach over 95%, and is much higher than that of a traditional extraction method.

Description

technical field [0001] The invention relates to a method for extracting DHA algae oil and algae protein from algae, belonging to the technical field of health care product preparation. Background technique [0002] DHA (chemically known as all-cis-4,7,10,13,16,19-docosahexaenoic acid) has important physiological functions for the human body. It is one of the main components of the human brain and retina, and plays an important role in the neurodevelopment of infants. In addition, DHA also has important functions such as lowering blood fat, reducing thrombosis, anti-atherosclerosis, delaying aging, and preventing cancer. [0003] The traditional DHA production process is mainly extracted from fish oil. However, due to the unstable source of fish oil and the disadvantages of fishy smell, more and more researchers and enterprises have carried out research and large-scale production of DHA by microbial fermentation. Among many DHA-producing microorganisms (including Schizochyt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/04C11B1/00C11B1/10C11B3/00
CPCC07K1/14C11B1/10C11B3/001C12P7/6472
Inventor 霍文严窦光朋张红波魏巍黎丽干昭波
Owner SHANDONG BAILONG CHUANGYUAN BIO TECH
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