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Preparation method of asymmetric vesicle

An asymmetric, vesicle technology, applied in the directions of microcapsules, capsule delivery, etc., can solve the problem of scarcity of asymmetric vesicle methods, and achieve the effects of uniform size, good repeatability and complete morphology

Active Publication Date: 2015-03-11
宜兴环保产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the existing methods for preparing asymmetric vesicles are scarce, and provide a method for preparing asymmetric vesicles

Method used

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  • Preparation method of asymmetric vesicle

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specific Embodiment approach 1

[0025] Specific embodiment 1: This embodiment is a method for preparing asymmetric vesicles, which is completed according to the following steps:

[0026] 1. Dissolve span80 in liquid paraffin to obtain an oil phase; use a sucrose aqueous solution with a concentration of 200mmol / L-500mmol / L as the water phase; inject the oil phase and the water phase into the microfluidic chip from different channel inlets mixed to obtain an emulsion;

[0027] The volume ratio of the quality of span80 described in step 1 to liquid paraffin is 0.01g:1mL;

[0028] The flow rate of the aqueous phase in the microfluidic chip described in step 1 is 1 μL / min;

[0029] The flow rate of the oil phase in step 1 in the microfluidic chip is 1 μL / min-5 μL / min;

[0030] 2. Mix the emulsion with the receiving liquid, and react at room temperature for 25min to 60min to obtain the mixed liquid A; then use the mixed solution of organic solvent and liquid paraffin to wash the mixed liquid A for 3 to 5 times t...

specific Embodiment approach 2

[0047] Embodiment 2: This embodiment differs from Embodiment 1 in that: the flow rate of the oil phase in step 1 in the microfluidic chip is 1 μL / min˜3 μL / min. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0048] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the flow rate of the oil phase in step 1 in the microfluidic chip is 3 μL / min˜5 μL / min. Other steps are the same as those in Embodiment 1 or 2.

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Abstract

The invention provides a preparation method of an asymmetric vesicle and relates to a preparation method of a vesicle. The invention aims to solve the problem of scarcity of a method for preparing the asymmetric vesicle. The preparation method comprises the following steps: mixing an oil phase with a water phase by using a micro-fluidic chip and obtaining emulsion; mixing the emulsion with receiving liquid, washing with a mixed solution of an organic solvent and liquid paraffin, adding an organic solvent containing DOPC (Dilinoleoylphosphatidylcholine) phospholipid, standing, adding a solution containing the asymmetric vesicle into a glucose aqueous solution, standing, removing the oil phase on the upper layer and finishing the preparation and the storage of the asymmetric vesicle. The asymmetric vesicle prepared by the preparation method is complete in appearance and uniform in size and has a size of 100-150 [mu]m. The invention can obtain the preparation method of the asymmetric vesicle.

Description

technical field [0001] The invention relates to a method for preparing vesicles. Background technique [0002] Vesicles are composed of spherical or ellipsoid single or multiple compartments formed by a closed bilayer. Vesicles formed from natural phospholipids are called liposomes. Since the structure of vesicles is very similar to that of cell membranes, they have been widely studied as biological membrane models. Utilizing the characteristic that the biomembrane can fuse with the cell membrane, vesicles have important application value in biomembrane simulation, immobilization and targeted release of drugs, synthesis of nanoparticles, and microreactors. Asymmetric vesicles are vesicle structures composed of different molecular layers (leaflets). Because of the asymmetry of molecular layers, they have important theoretical and practical significance in the fields of biomembranes, cells, and biomedicine. Contents of the invention [0003] The purpose of the present inv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50
Inventor 韩晓军张旭男
Owner 宜兴环保产业有限公司