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Tissue culture medium composition for adina rubella and rapid tissue culture method of adina rubella

A technology of fine-leaf water mass flower and tissue culture medium, which is applied in the directions of botanical equipment and methods, horticultural methods, and application, can solve problems such as no relevant reports, and achieve the effect of shortening the reproduction cycle and important application value.

Inactive Publication Date: 2015-03-25
ZHENGZHOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] P. chinensis belongs to woody plants, and its current propagation methods are mainly seeding and cutting. Up to now, no relevant reports have been found on the tissue culture and rapid propagation technology and plant regeneration method of P. spp.

Method used

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  • Tissue culture medium composition for adina rubella and rapid tissue culture method of adina rubella
  • Tissue culture medium composition for adina rubella and rapid tissue culture method of adina rubella
  • Tissue culture medium composition for adina rubella and rapid tissue culture method of adina rubella

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Experimental program
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Effect test

Embodiment 1

[0053] The tissue culture medium composition used in the present embodiment includes induction medium, proliferation medium and rooting medium;

[0054] The induction medium is used to induce the germination of the slender-leaved water cluster flower. Taking the preparation of 1L tissue culture medium as an example, the components are: No. 1 mother solution 10mL of MS medium + No. 2 mother solution 10mL of MS medium + No. 3 of MS medium Mother liquor 10mL+ MS medium No. 4 mother liquor 10mL+ inositol 100.0mg+VB 1 10.0 mg+VB 6 1.0mg + niacin 1.0 mg + coconut milk 20 mL + sodium hypochlorite 5 mg + 6-BA 3.0mg + NAA 1.0mg + agar 8g + sucrose 10g + fungicide, make up 1L of water, pH value is 5.8; fungicide: Eperon A agent 333μL + B dose 14.5 mg;

[0055] The proliferation medium is used to promote the formation of seedlings after germination. Taking the preparation of 1L tissue culture medium as an example, the components are: 10mL of MS medium No. 1 to No. 6 mother liquor + 6...

Embodiment 2

[0072] The tissue culture medium composition used in the present embodiment includes induction medium, proliferation medium and rooting medium;

[0073] The induction medium is used for inducing the germination of the water cluster flower of fine leaves. Taking the preparation of 1L tissue culture medium as an example, the components are No. 1 mother solution 10mL of MS medium+No. 2 mother solution 10mL of MS medium+No. 3 mother solution of MS medium 10mL+ MS medium No. 4 mother solution 10mL+ inositol 100.0mg+VB 1 10.0 mg+VB 6 1.0mg + Niacin 1.0 mg + Coconut Juice 20 mL + Sodium Hypochlorite 15 mg + 6-BA 6.0mg + NAA 3.0mg + Agar 11g + Sucrose 25g + Fungicide, make up 1L of water, pH value is 5.5; Bactericide: Eperon A agent 333μL + B dose 14.5 mg;

[0074] The proliferation medium is used to promote the formation of seedlings after germination. Taking the preparation of 1L tissue culture medium as an example, the components are: 10mL of MS medium No. 1 to No. 6 mother liq...

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Abstract

The invention belongs to the technical field of plant in-vitro tissue culture, and particularly relates to a tissue culture medium composition for adina rubella and a rapid tissue culture method of the adina rubella by utilizing the composition. The composition comprises an induction culture medium, a proliferation culture medium and a rooting culture medium, and is obtained by improving on the basis of an existing MS culture medium. The invention also provides an efficient and rapid tissue culture method of the adina rubella, so that the restriction of a traditional propagating method of the adina rubella is broken through; meanwhile, a great quantity of regeneration plants of the adina rubella can be obtained, and thus the propagating cycle is shortened greatly. According to data statistics, by an established rapid propagating system of the adina rubella, the inductivity of axillary buds reaches 80%, the induction coefficient is 4-6, the propagating coefficient is 8-10, the rooting rate reaches 100%, the cycle for generating the regeneration plants is 3-4 months, and the established rapid propagating system of the adina rubella has the relatively important application value to the popularization and the plantation of the adina rubella.

Description

Technical field [0001] The present invention belongs to the field of plant -lipped tissue training technology. It specifically involves a rapid organizational training method of a fine -leaf water group flower tissue culture medium and the fine leaf water group flowers that use the composition. Background technique [0002] Fine leaf water group flowers ( Adine rubella ) It is a small deciduous shrub in the Rubiaceae water group flower (Adina). Its branches are slender and scattered, with red brown pour.The leaves are raw, thick paper, non -reciprocating, ovate, lanceolate or ovate, dark green on the surface, and shiny.The head-shaped inflorescence is a single or 2-3 top or axillary; the flowers are purple.Film long ovate wedge.The flowering period is June-July, and the fruiting period is September to October.The branches of fine leaf water group flowers are scattered and pretty.The leaves are long and thick, green and glittering.When the flowers are blooming, the purple and red ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 蒋素华田云芳梁芳王林青马杰袁秀云崔波
Owner ZHENGZHOU NORMAL UNIV
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