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A nano-drug for controlling citrus Huanglongbing suitable for leaf spraying

A technology of citrus huanglongbing and nano-medicine, applied in the direction of chemicals, applications, animal repellents for biological control, etc., can solve the problem of not fundamentally eliminating citrus huanglongbing bacteria

Active Publication Date: 2016-06-29
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has not fundamentally eliminated the Huanglongbing bacteria in citrus

Method used

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  • A nano-drug for controlling citrus Huanglongbing suitable for leaf spraying
  • A nano-drug for controlling citrus Huanglongbing suitable for leaf spraying

Examples

Experimental program
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Effect test

Embodiment 1

[0020] The nano-medicine for preventing and treating citrus Huanglongbing that is suitable for leaf spraying according to the present invention, in parts by weight, the preparation raw materials are as follows: 100 parts of polyoxyethylene castor oil, 1 part of Span80, 90 parts of ethyl acetate, 1 part of ethyl acetate, 1 part of parts Tween 80, 10 parts 0.1 mg / ml ampicillin solution and 1000 parts deionized water containing 0.1% (w / w) Brij35.

[0021] The preparation method of above-mentioned nanometer medicine, comprises the steps:

[0022] (1) take by weight 100 parts of polyoxyethylene castor oil, 1 part of Span80 and 90 parts of ethyl acetate, then mix and stir to obtain homogeneous organic phase S1;

[0023] (2) take by weight 1 part of Tween80 and 10 parts of 0.1mg / ml ampicillin solution, then mix and stir to obtain homogeneous water phase S2;

[0024] (3) inject the organic phase S1 into the water phase S2, and stir for 30min, so that the components of the organic pha...

Embodiment 2

[0029] The nano-medicine for preventing and treating citrus Huanglongbing that is suitable for leaf spraying according to the present invention, in parts by weight, the preparation raw materials are as follows: 1000 parts of polyoxyethylene castor oil, 100 parts of Span80, 1800 parts of ethyl acetate, 100 parts of ethyl acetate, 100 parts of polyoxyethylene castor oil Parts Tween80, 1000 parts of 2.0 mg / ml ampicillin solution and 250000 parts of 0.5% (w / w) Brij35 in deionized water.

[0030] The preparation method of above-mentioned nanometer medicine, comprises the steps:

[0031] (1) take by weight 1000 parts of polyoxyethylene castor oil, 100 parts of Span80 and 18 parts of ethyl acetate, then mix and stir to obtain homogeneous organic phase S1;

[0032] (2) take by weight 100 parts of Tween80 and 1000 parts of 2.0mg / ml ampicillin solutions, then mix and stir to obtain homogeneous water phase S2;

[0033] (3) The organic phase S1 was injected into the water phase S2, and s...

Embodiment 3

[0038] The nanometer medicine for preventing and treating citrus Huanglongbing which is suitable for leaf spraying according to the present invention, in parts by weight, the preparation raw materials are as follows: 300 parts of polyoxyethylene castor oil, 5 parts of Span80, 200 parts of ethyl acetate, 5 parts of ethyl acetate, 5 parts of parts Tween 80, 200 parts of a 0.2 mg / ml solution of ampicillin and 30000 parts of a deionized aqueous solution containing 0.4% (w / w) Brij35.

[0039] The preparation method of above-mentioned nanometer medicine, comprises the steps:

[0040] (1) take by weight 300 parts of polyoxyethylene castor oil, 5 parts of Span80, 200 parts of ethyl acetate, then mix and stir to obtain homogeneous organic phase S1;

[0041] (2) take by weight the 0.2mg / ml ampicillin solution of 5 parts of Tween80 and 200 parts, then mix and stir to obtain homogeneous water phase S2;

[0042] (3) inject the organic phase S1 into the water phase S2, stir for 50min, make...

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Abstract

The invention provides a nano drug applicable to leaf spraying to prevent and treat citrus huanglongbing. The nano drug comprises the following preparation raw materials in parts by weight: 100-1000 parts of polyoxyethylene castor oil, 1-100 parts of Span 80, 90-1800 parts of ethyl acetate, 1-100 parts of Tween 80, 10-1000 parts of 0.1mg / ml-2.0mg / ml ampicillin and 1000-250000 parts of a de-ionized water solution containing Brij 35. A preparation method comprises the following steps: injecting an organic phase into a water phase; stirring to uniformly disperse all components of the organic phase into the water phase; stirring under ultrasonic wave so as to balance a system to form water-in-oil nano-emulsion; carrying out reduced pressure distillation to completely remove the organic solvents in the system; and dissolving a distilling mother solution into the de-ionized water solution containing Brij 35. According to the formula of the drug, the drug can relatively effectively penetrate through leaf surfaces so that huanglongbing pathogenic bacteria in the citruses can be effectively removed.

Description

technical field [0001] The invention relates to a medicine for preventing and treating fruit tree diseases, in particular to a nano-medicine suitable for leaf spraying for preventing and treating citrus huanglongbing. Background technique [0002] Citrus Huanglongbing is a devastating disease for world citrus production. According to relevant studies, it is estimated that hundreds of millions of citrus trees in more than 40 countries in the world are infected with citrus Huanglongbing. In my country, citrus Huanglongbing was first reported in southern China in the early 20th century, and it has severely restricted the development of the citrus industry in Guangdong, Guangxi and Fujian for more than a century. Taiwan, Zhejiang, Jiangxi, Hunan, Guizhou, Hainan, Yunnan, Sichuan and some areas adjacent to Yunnan have also reported the occurrence and damage of Huanglongbing disease. The affected area accounts for more than 80% of the total citrus cultivation area, and the output...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01N25/04A01P1/00
Inventor 张木清杨川毓
Owner GUANGXI UNIV
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