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Melon pollen cryopreservation method

A technology for ultra-low temperature preservation and melon flowers, which is applied in the direction of plant preservation, botany equipment and methods, applications, etc., can solve the problems of inability to preserve the activity of melon pollen, inapplicable melon pollen, complicated operation, etc., and achieve the purpose of inhibiting parental outflow, The effect of short drying time and simple and easy operation

Inactive Publication Date: 2015-04-08
河北双星种业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, there is no research on the storage of melon pollen, and the cryopreservation of melon pollen by the method of preserving similar species in the existing technology cannot preserve the activity of melon pollen.
The commonly used drying degree is 8-13%, and usually needs to be thawed gradually, the operation is complicated, and it is not suitable for melon pollen

Method used

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  • Melon pollen cryopreservation method
  • Melon pollen cryopreservation method
  • Melon pollen cryopreservation method

Examples

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Embodiment 1

[0029] The method for the cryopreservation of the muskmelon pollen of the present embodiment is carried out in the following steps:

[0030] 1. Deflowering: Pick the freshly opened melon flowers at the Shijiazhuang Seed Production Base between 6:00 am and 8:00 am in May and June;

[0031] 2. Collecting pollen and drying: collect pollen in a cool and dry place with a temperature of 25°C and a humidity of 40%, and place the collected pollen on kraft paper for 1 hour;

[0032] 3. Pre-freezing: put the pollen treated in step 2 into a cryopreservation tube, and pre-freeze at -10°C for 1 hour;

[0033] 4. Storage: store the cryopreservation tube in liquid nitrogen for one year;

[0034] 5. Thawing: Take out the stored pollen from liquid nitrogen, spread it on kraft paper, and place it at room temperature for 10 minutes.

Embodiment 2

[0036] The method for the cryopreservation of the muskmelon pollen of the present embodiment is carried out in the following steps:

[0037] 1. Deflowering: Pick the freshly opened melon flowers at the Shijiazhuang Seed Production Base between 6:00 am and 8:00 am in May and June;

[0038] 2. Collecting pollen and drying: collect pollen in a cool and dry place with a temperature of 25°C and a humidity of 40%, and place the collected pollen on kraft paper for 1 hour;

[0039] 3. Pre-freezing: put the pollen treated in step 2 into a cryopreservation tube, and pre-freeze at -10°C for 1 hour;

[0040] 4. Storage: Place the cryopreservation tube in the powder or granular dry ice in the incubator for 7 days (replenish dry ice according to the volatilization of dry ice during the period);

[0041] 5. Thawing: Take out the stored pollen from liquid nitrogen, spread it on kraft paper, and place it at room temperature for 10 minutes.

[0042] In this embodiment, the melon pollen is sto...

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Abstract

The invention discloses a melon pollen cryopreservation method, relates to a local plant preservation method, and in particular relates to a melon pollen preservation method for long-term preservation. The invention aims at providing a melon pollen cryopreservation method which is easy and convenient to operate, effective and feasible. The melon pollen cryopreservation method disclosed by the invention comprises the following steps: picking flowers, collecting pollen, drying, pre-freezing, storing and unfreezing. The melon pollen cryopreservation method disclosed by the invention is simple and easy to operate and can be used for seed production. The method disclosed by the invention is applied to the field of melon pollen preservation.

Description

technical field [0001] The invention relates to a method for preserving plant parts, in particular to a method for preserving melon pollen for long-term preservation. Background technique [0002] With the development of economy and the destruction of ecological environment, germplasm resources are constantly lost and extinct. How to protect the existing plant germplasm and maintain its diversity has attracted global attention. [0003] As a form of plant germplasm, pollen contains various gene types of the plant and is the main material for germplasm preservation and exchange. Since 1960, the Food and Agriculture Organization of the United Nations (FAO) and the International Commission on Plant Genetic Resources (IBPGR) have been actively supporting scientific research institutions in relevant countries to carry out pollen conservation work. However, the pollen life is very short, and the survival time at room temperature after leaving the plant is not long. The success o...

Claims

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Application Information

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IPC IPC(8): A01N3/00
Inventor 范冬冬党继革代云志李涛吴涛赵翠媛高雪萍张慧
Owner 河北双星种业股份有限公司
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