The method of cryopreservation of sunflower pollen

An ultra-low temperature preservation, sunflower technology, applied in the fields of plant preservation, botanical equipment and methods, application, etc., can solve the problem of rare long-term storage of sunflower pollen, and achieve the effect of inhibiting parental outflow and being simple and easy to operate.

Active Publication Date: 2016-03-23
河北双星种业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few reports on the long-term storage of sunflower pollen, and most of them can only be stored for a few days

Method used

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  • The method of cryopreservation of sunflower pollen
  • The method of cryopreservation of sunflower pollen
  • The method of cryopreservation of sunflower pollen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The method for the ultra-low temperature preservation of sunflower pollen of the present embodiment is carried out in the following steps:

[0022] 1. Collect pollen: collect the pollen on the flower disc at 10 am in Shijiazhuang area, and pass the collected pollen through a 40-mesh sieve to remove impurities;

[0023] 2. Determination of pollen activity: the pollen activity was measured by pollen in vitro germination method, and the obtained pollen activity was 76%;

[0024] 3. Drying: spread the pollen on kraft paper, and dry it in a cool place at room temperature until the water content of the pollen is 81%;

[0025] 4. Cryopreservation: put the dried pollen into a cryopreservation tube, place it in liquid nitrogen for 10 seconds, and then transfer it to a -80°C refrigerator for storage;

[0026] 5. Thawing: After taking out the stored pollen from the -80°C refrigerator, put it directly on kraft paper at room temperature to thaw for 0.5h.

Embodiment 2

[0028] The method for the ultra-low temperature preservation of sunflower pollen of the present embodiment is carried out in the following steps:

[0029] 1. Collect pollen: Collect the pollen on the flower disc at 11 am in Shijiazhuang area, and pass the collected pollen through a 50-mesh sieve to remove impurities;

[0030] 2. Determination of pollen activity: the pollen activity was measured by pollen in vitro germination method, and the obtained pollen activity was 71%;

[0031] 3. Drying: spread the pollen on kraft paper, and dry it in a cool place at room temperature until the water content of the pollen is 90%;

[0032] 4. Cryopreservation: put the dried pollen into a cryopreservation tube, put it in liquid nitrogen for 5 seconds, and then transfer it to a -80°C refrigerator for storage;

[0033] 5. Thawing: After taking out the stored pollen from the -80°C refrigerator, put it directly on kraft paper at room temperature to thaw for 0.5h.

Embodiment 3

[0035] The method for the ultra-low temperature preservation of sunflower pollen of the present embodiment is carried out in the following steps:

[0036] 1. Collect pollen: Collect the pollen on the flower disc at 9 am in Hainan area, pass the collected pollen through a 60-mesh sieve to remove impurities;

[0037] 2. Determination of pollen activity: the pollen activity was measured by the pollen in vitro germination method, and the obtained pollen activity was 55%;

[0038] 3. Drying: spread the pollen on kraft paper, and dry it in a cool place at room temperature until the water content of the pollen is 86%;

[0039] 4. Cryopreservation: Put the dried pollen into a cryopreservation tube, put it in liquid nitrogen for 8 seconds, and then transfer it to a -80°C refrigerator for storage;

[0040] 5. Thawing: After taking out the stored pollen from the -80°C refrigerator, put it directly on kraft paper at room temperature to thaw for 0.5h.

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Abstract

The invention discloses an ultra-low temperature storage method for sunflower pollen, relates to a local storage method for plants, and in particular relates to a method for storing the sunflower pollen for a long time. The invention aims to provide ultra-low-temperature storage method for sunflower pollen, which is convenient for long-distance transportation and storage, and a matched pollen in-vitro germination cultivation medium. The ultra-low-temperature storage method for sunflower pollen comprises the following steps: collecting pollen; measuring pollen activity; drying; freezing; unfreezing. The ultra-low temperature storage method for sunflower pollen disclosed by the invention is simple and easy to operate, and can be used for producing and preparing seeds. The ultra-low temperature storage method is applied to the storage field of melon pollen.

Description

technical field [0001] The invention relates to a method for preserving plant parts, in particular to a method for long-term preservation of sunflower pollen. Background technique [0002] Sunflower originated in North America. It is a plant of Compositae and Helianthus. The seeds of sunflower have high economic value. Not only can they be made into popular sunflower seeds, but also high-grade edible sunflower oil with low cholesterol can be squeezed out. Sunflower has been cultivated in my country for decades. It is one of the important economic crops in more than 20 provinces, municipalities and autonomous regions. [0003] Sunflower has obvious heterosis, and most of the sunflower seeds used in production are the first generation of hybrids. In the "three-line" breeding work, sometimes it is necessary to use pollen from other places, and sometimes the problem of parental sterility during flowering may be encountered. Only by breaking the time and space isolation of hybr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N3/00
Inventor 范冬冬党继革代云志李涛赵翠媛高雪平张慧
Owner 河北双星种业股份有限公司
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