A kind of seed anti-aging agent and preparation method thereof

An anti-aging agent and anti-aging technology, applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems of prolonging the shelf life of seeds, and achieve easy control of operating process conditions, high efficiency, and simple methods Effect

Inactive Publication Date: 2017-08-25
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a seed anti-aging agent for delaying seed aging and a method for using the anti-aging agent to prevent seed aging in view of the existing technical problems of maintaining seed vitality and preventing seeds from aging during storage. The method of the present invention This anti-aging agent can effectively prevent the aging of seeds, and has a very significant effect on delaying the aging of seeds caused by high temperature and humidity, and can effectively prolong the shelf life of seeds

Method used

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  • A kind of seed anti-aging agent and preparation method thereof
  • A kind of seed anti-aging agent and preparation method thereof
  • A kind of seed anti-aging agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043]The seeds of elm tree, sea buckthorn, rapeseed, Haloxylon ammonium, Alfalfa, Arborvitae, Caragana intermedia, Sand Holly, Albizia Julibrissin newly harvested every year are placed on a germination bed for germination treatment, and the germination bed is a petri dish with a diameter of 12 cm. Place a double layer of filter paper at the bottom, moisten it with water, put 50 seeds in each dish, 4 repetitions; place the germination bed in a light incubator at a temperature of 25±1°C, a light intensity of 80Lux, and a light and dark time of 12h each. The germination treatment of the seeds was carried out, and the germination rate was recorded every day until there was no germination. The calculation results are shown in Table 1-9.

Embodiment 2

[0045] 1. Sterilization of seeds

[0046] The seeds (elm) were first soaked in HgCl with a concentration of 0.1% by mass 2 The solution was sterilized for 10 minutes, then taken out, dried at room temperature (20-30° C.) to the original water content of 7.8%, and used for later use.

[0047] 2. Preparation of anti-aging agent

[0048] 2-1) Add 75 mmol of cyclosporine A (CsA for short) to 1 L of dimethyl sulfoxide (DMSO), stir evenly, and make a CsA-DMSO solution with a concentration of 75 mM, that is, the anti-aging agent mother liquor, Then store at -20°C for future use;

[0049] In addition to the concentration of the anti-aging agent stock solution of the present invention of 75 mM, the CsA-DMSO solution with a concentration of 50-150 mM is also suitable for the present invention.

[0050] 2-2) Mix the anti-aging agent mother liquor with the osmotic adjusting liquid, wherein the volume ratio of the anti-aging agent mother liquid and the osmotic adjusting liquid is 1:500,...

Embodiment 3

[0066] Except for soaking the seeds with distilled water in the "seed anti-aging treatment" step, the rest is the same as that in Example 2, and the calculation results of the seed germination rate are shown in Table 1.

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Abstract

The invention discloses a seed anti-aging agent, its preparation method and a method for treating seeds with the seed anti-aging agent. The seed anti-aging agent prepared by the method of the invention includes cyclosporine A. The anti-aging agent of the invention can effectively It can effectively maintain the vitality of seeds, significantly prevent the aging of seeds, and has a very significant effect on delaying the aging of seeds caused by high temperature and humidity, and can effectively prolong the shelf life of seeds.

Description

technical field [0001] The invention relates to a pharmacological agent capable of delaying the aging of seeds, in particular to an anti-aging seed treatment agent. It can be used for the storage of endangered plant seeds. Background technique [0002] The seed vigor is formed during the development of the seed, but the seed undergoes an irreversible deterioration process of decreasing vigor since it matures, that is, the aging of the seed. The aging and deterioration of seeds is a common phenomenon in nature, and it is also a serious problem in the preservation of germplasm resources. How to effectively prolong the storage life of seeds and improve the vitality of seeds is an important issue of the national economy. [0003] In the mid-1970s, Heydeekert et al. first used PEG to initiate experiments on onion seeds, and obtained the effect of uniform emergence. Song Hong and Wang Danhong et al. (1993) pointed out that H 2 O 2 Seed soaking can improve the germination rate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N3/00A01N43/00A01P21/00
Inventor 汪晓峰王昱李莹
Owner BEIJING FORESTRY UNIVERSITY
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