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Purification method of grifolan

A technology of Grifola frondosa polysaccharide and purification method, which is applied in the field of purification of biologically active substances, can solve the problems of polysaccharide yield and purity limitations, large amount of organic reagents, unfavorable polysaccharide products, etc., and achieve great development significance, good product quality, and easy operation convenient effect

Inactive Publication Date: 2015-04-08
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently, Grifola frondosa polysaccharide extract products are rare at home and abroad. Most of the literatures use water extraction and alcohol precipitation process to extract and remove impurities such as protein and pigment, or use traditional extraction methods such as hot water extraction, acid extraction, etc. Extraction, alkali extraction, etc. combined with general deproteinization purification methods such as alcohol precipitation, Sevage, trichloroacetic acid, etc. to purify polysaccharides have disadvantages such as long operation time, large amount of organic reagents, low efficiency, and poor effect. Yield and purity are greatly limited, which is not conducive to industrial production and preparation of polysaccharide products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Extraction and purification of polysaccharides from Grifola frondosa:

[0028] (1) Crush and sieve the dried Grifola frondosa, accurately weigh 3 g of the medicinal material with a particle size of 20 mesh, place it in a 250 mL three-necked flask, add 90 mL of deionized water, weigh, extract with microwave for 210 seconds, cool to room temperature, and then Weigh and rehydrate, and suction filter to obtain the extract of Grifola frondosa polysaccharide.

[0029] (2) Weigh 200g of HD-8 resin, soak it with deionized water repeatedly for 5 times, and change the water every 15 minutes while stirring from time to time, and then change the water every 30 minutes, and soak twice. Then soak in 400mL, 1mol / L NaOH for 24h, soak in deionized water 7 times, then soak in 400mL, 1mol / L hydrochloric acid for 24h, and soak in deionized water 8 times.

[0030] (3) Weigh 20g of the pretreated resin for wet packing, inject about 40mL of deionized water into the chromatography column (1.5...

Embodiment 2

[0035] Extraction and purification of polysaccharides from Grifola frondosa:

[0036] (1) Crush and sieve the dried Grifola frondosa, accurately weigh 3g of the medicinal material with a particle size of 20 mesh, place it in a 250mL three-neck flask, add 90mL of deionized water, weigh, extract by microwave for 180s, cool to room temperature, and then Weigh and rehydrate, and suction filter to obtain the extract of Grifola frondosa polysaccharide.

[0037] (2) Weigh 200g of HD-8 resin, soak it with deionized water repeatedly for 5 times, and change the water every 15 minutes while stirring from time to time, and then change the water every 30 minutes, and soak twice. Then soak in 400mL, 1mol / L NaOH for 24h, soak in deionized water 7 times, then soak in 400mL, 1mol / L hydrochloric acid for 24h, and soak in deionized water 8 times.

[0038] (3) Weigh 20g of the pretreated resin for wet packing, inject about 40mL of deionized water into the chromatography column (1.5×50cm), and dr...

Embodiment 3

[0043] Extraction and purification of polysaccharides from Grifola frondosa:

[0044] (1) Crush and sieve the dried Grifola frondosa, accurately weigh 3 g of the medicinal material with a particle size of 20 mesh, place it in a 250 mL three-necked flask, add 90 mL of deionized water, weigh, extract with microwave for 210 seconds, cool to room temperature, and then Weigh and rehydrate, and suction filter to obtain the extract of Grifola frondosa polysaccharide.

[0045](2) Weigh 200g of HD-8 resin, soak it with deionized water repeatedly for 5 times, and change the water every 15 minutes while stirring from time to time, and then change the water every 30 minutes, and soak twice. Then soak in 400mL, 1mol / L NaOH for 24h, soak in deionized water 7 times, then soak in 400mL, 1mol / L hydrochloric acid for 24h, and soak in deionized water 8 times.

[0046] (3) Weigh 20g of the pretreated resin for wet packing, inject about 40mL of deionized water into the chromatography column (1.5×...

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Abstract

The invention relates to a purification method of grifolan. The purification method comprises the following steps: grinding grifola frondosa and carrying out microwave extraction by taking deionized water as a solvent; carrying out deproteinization purification by HD-8 cation exchange resins; diluting or concentrating the polysaccharide extraction solution to load sample; controlling a certain flow velocity; collecting the eluate to obtain the high-purity bioactivator grifolan purified solution. The method is short in process, simple and convenient to operate, high in efficiency and less in energy consumption, and has the advantages of avoiding the consumption of plenty of organic reagents, serializing the separation and purification process, recycling the resins to bring safety and environmental friendliness and being low in cost of preparation process, beneficial for reducing the product cost and suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to a method for purifying biologically active substances from edible fungi, in particular to a method for purifying Grifola frondosa polysaccharides using a microwave extraction-HD-8 cation exchange resin purification integration process. Background technique [0002] Grifola frondosa, also known as chestnut mushroom, lotus fungus, thousand Buddha fungus, etc., is a precious medicinal and edible fungus with delicious taste and rich in various biologically active substances, minerals and vitamins. Its main bioactive substance, Grifola frondosa polysaccharide, has been a research hotspot in recent years. [0003] Numerous pharmacological and clinical tests have confirmed that Grifola frondosa polysaccharide has anti-tumor, lowering blood pressure, regulating blood sugar and blood lipid levels, anti-HIV virus, and enhancing the function of the immune system, especially the anti-tumor activity. Japanese medical experts have found that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 韩伟满宁罗文锋韩乐马迪豆欣欣汪佳丹
Owner EAST CHINA UNIV OF SCI & TECH
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