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Method for quickly detecting content of glycogen and protein of crassostrea gigas

A technology for protein content and long oysters, which is applied to measurement devices, material analysis by optical means, instruments, etc., can solve the problems of low efficiency, high detection cost, long time consumption, etc., and achieves low experimental cost, wide application prospects, and testing fast effect

Inactive Publication Date: 2015-04-08
山东省海洋资源与环境研究院
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  • Application Information

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Problems solved by technology

However, traditional glycogen and protein chemical detection methods have the disadvantages of long time consumption, low efficiency and high detection cost.

Method used

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  • Method for quickly detecting content of glycogen and protein of crassostrea gigas
  • Method for quickly detecting content of glycogen and protein of crassostrea gigas

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Experimental program
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Embodiment Construction

[0019] Materials and Methods

[0020] 1.1 Main instruments and equipment

[0021] Fourier transform NIR spectrometer (Antaris MX, USA), equipped with RESULT TM Integrated software for sample spectral collection and data processing software TQ analyst (Thermo Fisher, USA), microplate reader (BIO-RAD, USA), atomic fluorescence spectrometer (PA-10) and microwave digestion system (Mars Xpress, USA), high-efficiency Liquid chromatography (Waters Inc., USA), flame atomic absorption spectrophotometer (AA-800, USA), automatic Kjeldahl nitrogen analyzer (FOSS kjeltec TM 2300, Sweden), Soxhlet extractor, muffle furnace and large vacuum freeze dryer (ZDGX5), homogenizer (IKA ? T18 basic ULTRA-TURRAX ? , Germany).

[0022] sample collection

[0023] During the period from November 2012 to October 2013, the oysters were collected from 7 places in Rushan, Zhifu Island, Kongtong Island and Liugong Island in Shandong, Donggang and Xiaoshan Island in Liaoning, and Ganyu in Jiangsu. A t...

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Abstract

The invention discloses a method for quickly detecting the content of glycogen and protein of crassostrea gigas. The method comprises the following steps: firstly, homogenizing a to-be-detected sample; then, collecting near infrared spectrum data; and finally comparing with a pre-established crassostrea gigas glycogen and protein content near infrared model to acquire a measurement value of the to-be-detected sample. The method has the characteristics of high detection speed, no chemical reagent, low experiment cost and no environmental pollution in the process of analyzing the content of glycogen and protein of crassostrea gigas. Establishment of the method for quickly detecting content of glycogen and protein of crassostrea gigas has important meaning to crassostrea gigas meat quality analysis, meat property breeding, breeding generation identification and genetic resource evaluation.

Description

technical field [0001] The invention relates to a method for the nutritional components of shellfish, in particular to a method for rapidly detecting the glycogen and protein content of long oyster. Background technique [0002] long oysters ( Crassostreagigas ), also known as Pacific oyster, has the advantages of strong environmental adaptability, rapid growth, and rich nutrition. It is the world's most widely distributed bulk economic shellfish with the highest yield. In 2012, the total output of oysters in my country reached 3.95 million tons, ranking first in the world, providing people with a rich source of protein. [0003] Different flavors and nutritional qualities of oysters affect consumers' consumption tendency, which in turn determines their commodity value. With the continuous improvement of living standards, people pay more attention to the flavor and nutrition of the meat when consuming oysters, and the glycogen content and protein content in oysters affect ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359
Inventor 王卫军杨建敏冯艳微韦秀梅孙国华李彬
Owner 山东省海洋资源与环境研究院
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