Photometric analyzer based on light guide metal capillary and detection method thereof

Abstract

The invention relates to a photometric analyzer based on a light guide metal capillary and a detection method thereof. The analyzer comprises a light source, a light detector and the light guide metal capillary, and is characterized in that a light wave can be reflected on the side wall of the light guide metal capillary, a detection light beam emitted by the light source is restricted in the light guide metal capillary for transmission through total reflection on the side wall of the light guide metal capillary, and the light detector is used for receiving the detection light beam emitted out from the light guide metal capillary; a sample inlet is formed in one end of the light guide metal capillary, and a sample outlet is formed in the other end of the light guide metal capillary. The analyzer utilizes the light guide metal capillary to guide the transmission of the detection light beam and a sample to be detected; the total reflection for light on a metal surface is not limited by critical angle, and corrugated fluctuation on the inner surface of the metal capillary enables the incident angle of the detection light beam to change, so that the transmission light path of the detection light beam in the sample can be greatly increased, and the detection precision is improved.

Description

technical field [0001] The invention relates to a photometric analyzer and a detection method thereof, in particular to a photometric analyzer based on a light-guiding metal capillary and a detection method thereof, which can be used for absorbance testing of liquid and gas samples. Background technique [0002] Absorbance is an important material characteristic parameter. The absorbance of a sample can be used to obtain relevant information such as the composition and concentration of the sample, and optical detection has the characteristics of anti-electromagnetic interference and fast response. Therefore, spectrophotometers (or colorimeters) based on absorbance detection are widely used in the field of biochemical analysis. For a spectrophotometer or a colorimeter, it is necessary to place the sample to be tested and the standard sample in different cuvettes during the test, and perform a comparative test by changing the cuvette (Sensors and Actuators B, 191, 561-566 (201...

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Problems solved by technology

Therefore, the existing test method usually needs to change the cuvette back and forth, which increases the test time and reduces the test accuracy.

In addition, in order to improve the test accuracy, it is necessary to increase the thickness of the cuvette (that is, to increase the absorption length of the sample to the probe light), which increases the volume of the cuvette and the amount of sample required

Although the patent CN201210105716.9 discloses the use of capillary in the field of colorimetric analysis, there are the following problems: when the light source passes through the capillary, the light source may diverge and pass through the capillary as the distance of the light path increases; The optical path deviates from the centerline of the capillary, and the light source passes through the capillary, which will seriously reduce the test accuracy

The disadvantages of this structure: if the film is coated on the inner wall of the capillary, the process is very complicated; if the metal film is coated on the outer wall of the capillary, since the refractive index of glass is larger than that of water, the light wave tends to be in the measurement wall of the quartz tube Transmission, rather than transmission in the liquid, so it will reduce the detection accuracy; in addition, the metal surface formed by the coating has low smoothness and density, so it has a large scattering of light and a large transmission loss

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