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Sample clamping tool and transfer box for frozen water-containing slices prepared by focused ion beam method

A clamping tool and sample transfer technology, used in the preparation of test samples, sampling devices, etc., can solve the problems of not having a wide range of applications, easy damage to the carrier, no products, etc., to reduce ice pollution, easy to use, prevent Effects of Ice Crystal Pollution

Active Publication Date: 2015-04-29
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The clamping and transport devices used in the research of the above research groups are all developed by themselves based on the special transmission electron microscope of this research group. The carrier grid is easily damaged and does not have wide application properties.
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Method used

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  • Sample clamping tool and transfer box for frozen water-containing slices prepared by focused ion beam method
  • Sample clamping tool and transfer box for frozen water-containing slices prepared by focused ion beam method
  • Sample clamping tool and transfer box for frozen water-containing slices prepared by focused ion beam method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Preparation of samples

[0039] Use the throwing freezing technique to quickly freeze the sample onto a 200-mesh molybdenum grid. If cell samples are involved, it is recommended to use the method of dripping the sample on the front of the grid and drying it from the back to ensure that the ice layer of the sample is thin.

[0040] 2. Cutting process

[0041] 1. In a liquid nitrogen environment, install the grid on the holding tool, then transfer the sample holding tool loaded with the grid to the sample transfer box, and close the protective cover.

[0042] 2. Transfer the sample holding tool to the PP3000T large sample preparation cup and connect it to the sample transfer rod, and apply a vacuum.

[0043] 3. After the sample enters the PP3000T sample preparation chamber, it is sprayed with Pt metal and then transferred to the SEM low-temperature sample stage of the scanning electron microscope.

[0044] 4. Use the focused ion beam thinning method to thin and cut t...

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Abstract

The invention discloses a set of sample clamping tool and transferring box for preparing biological frozen moisture contained chips by a focused ion beam method. The set of sampling clamping tool and transferring box comprises a sample clamping device and a transferring device. The innovation of the set of sample clamping tool and transferring box lies in the fact that ice pollution caused to biological frozen moisture contained samples during preparation and transportation of chips can be effectively lowered, and the cutting thicknesses of the prepared sample chips are less than 300nm. The samples prepared by the devices are suitable for imaging of an ordinary electron microscopy with a directly mounted carrying net and imaging of a transmission electron microscopy with a special requirement on a carrying net.

Description

technical field [0001] The invention relates to the fields of cryo-electron microscope electron tomography sample preparation and in-situ transmission electron microscope sample preparation. Background technique [0002] Cryo-electron tomography three-dimensional reconstruction (cryo-ET) technology is an important technology for the development of structural biology and cell biology research. This technology can obtain a more real and close to natural high-resolution three-dimensional structure of cells and information on the location and interaction of protein macromolecules, and is an important auxiliary means for proteomics research. The commonly used sample preparation method --- cryosection technology, has many problems, such as sample compression, vibration, shrinkage, fracture and poor grid adhesion during sectioning and collection. These defects have seriously affected this research field. development of. In recent years, a new method for preparing frozen water-con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/42G01N1/04
Inventor 张建国季刚徐伟孙飞
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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