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Detection method of pharmaceutical composition Xianyu for treating epileptoid convulsions, infantile convulsions and facial spasms

A technology for infantile convulsions and detection methods, applied in drug combinations, pharmaceutical formulas, measuring devices, etc., can solve problems such as inability to reflect drug quality, poor activity correlation, and poor specificity

Active Publication Date: 2015-04-29
XIAN CHIHO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Protocatechualdehyde exists in a variety of medicinal materials, but it is not an index specific component of Danshen, and has poor correlation with activity
Therefore, the qualitative identification of Salvia miltiorrhiza using protocatechualdehyde as an index component has poor specificity and cannot reflect the quality of the drug.
In addition, there are few quantitative determination items in the prior art, for example, there is no quantitative detection of the main components Gastrodia elata and Radix Astragalus

Method used

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  • Detection method of pharmaceutical composition Xianyu for treating epileptoid convulsions, infantile convulsions and facial spasms
  • Detection method of pharmaceutical composition Xianyu for treating epileptoid convulsions, infantile convulsions and facial spasms
  • Detection method of pharmaceutical composition Xianyu for treating epileptoid convulsions, infantile convulsions and facial spasms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Example 1 A capsule for the treatment of epileptic convulsions, infantile convulsions, and hemifacial spasms (Xianyu Capsules)

[0106] 1. Prescription of traditional Chinese medicine raw materials: Astragalus 140g, Codonopsis 140g, Salvia 140g, Bupleurum 70g, Semen jujube 105g, Polygala 70g, Gastrodia elata 105g, Uncaria 105g, Shichangpu 105g, Dannanxing 70g, Angelica 140g, Bombyx mori 105g, Liushenqu 70g, turmeric 70g, licorice 70g, white aconite 35g, made into 1000 capsules in total.

[0107] 2. Preparation method: The above sixteen flavors, silkworm, and Liu Shenqu are crushed into fine powder, sieved, and set aside; the other fourteen flavors such as astragalus are boiled in water for three times, the first time for 2 hours, the second time for 1.5 hours, and the third time After 1 hour, combine the decoction, filter, and concentrate the filtrate under reduced pressure to a thick paste with a relative density of 1.36 (60°C), add the above-mentioned fine powder, m...

Embodiment 2

[0108] Example 2 Detection of Gastrodin by Liquid Chromatography

[0109] 1. Methodological research

[0110] 1. Selection of measurement wavelength:

[0111] Weigh an appropriate amount of gastrodin reference substance, dissolve it in a mixture of acetonitrile-0.05% phosphoric acid solution (3:97), and perform spectral scanning in the range of 200-900nm. As a result, gastrodin has a maximum absorption at a wavelength of 220nm, which is consistent with the Chinese Pharmacopoeia 2010 edition Consistent, so the detection wavelength is selected as 220nm.

[0112] 2. Chromatographic conditions and system suitability test:

[0113] Octadecylsilane bonded silica gel (water-resistant type) is used as the filler; the mobile phase is composed of acetonitrile and 0.05% phosphoric acid aqueous solution with a weight-to-volume ratio; the flow rate is 0.6ml / min; the detection wavelength is 220nm. The volume ratio of acetonitrile and 0.05% phosphoric acid aqueous solution in the mobile...

Embodiment 3

[0169] Example 3 Determination of Astragaloside IV by Liquid Chromatography

[0170] 1. Methodological research

[0171] 1. Chromatographic conditions and system suitability experiment:

[0172] Octadecylsilane bonded silica gel is used as filler; acetonitrile-water is used as mobile phase; evaporative light scattering detector is used for detection. The mobile phase and evaporative light scattering detector drift tube temperature were investigated and the results are shown in Tables 9 and 10.

[0173] Table 9 Investigation of mobile phase ratio

[0174]

[0175] Table 10 Drift tube temperature inspection

[0176]

[0177]

[0178] The results in Table 9 show that when the volume ratio of acetonitrile-water is 35:65, the resolution of astragaloside IV is the highest, the number of theoretical plates calculated as astragaloside IV is high, and the retention time is moderate. Therefore, the preferred mobile phase is acetonitrile-water in a volume ratio of 35:65. ...

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Abstract

The invention provides a detection method of pharmaceutical composition Xianyu for treating epileptoid convulsions, infantile convulsions and facial spasms. The detection method comprises steps as follows: detecting the content of gastrodin in the pharmaceutical composition with a liquid chromatography; detecting the content of astragaloside in the pharmaceutical composition with an HPLC (high performance liquid chromatography); detecting salvianic acid A sodium in the pharmaceutical composition with a TLC (thin-layer chromatography). With the adoption of the detection method, the quality of the pharmaceutical composition can be controlled more accurately and can be stable, controllable, efficient and safe, defects in the prior art can be overcome, and the powerful guarantee can be provided for well satisfaction of medical requirements.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and in particular relates to a method for detecting a pharmaceutical composition for treating epileptic convulsions, infantile convulsions, and hemifacial spasm, in particular to a capsule for treating epileptic convulsions, infantile convulsions, and hemifacial spasm ( Xianyu Capsule) detection method. Background technique [0002] Epilepsy capsules are composed of astragalus, codonopsis, salvia, bupleurum, jujube seed, polygala, gastrodia elata, uncaria, shichangpu, dannanxing, angelica, silkworm, Liushenqu, turmeric, licorice, and white aconite. It consists of dispelling phlegm and resuscitation, calming the nerves and calming convulsions, quenching wind and relieving spasm, and is used for the treatment of epileptic convulsions, infantile convulsions, and hemifacial spasm caused by wind-phlegm obstruction. All the medicines in the prescription have a synergistic effect and can achieve the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/90A61K36/9066A61K9/48A61P25/08A61P21/02
Inventor 胡小虎吕慧锋
Owner XIAN CHIHO PHARMA
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