Prevention of fibroblast collapse
A skin care composition, a technology of oleracea, applied in the field of personal care compositions, which can solve the problems of not considering the effects of cell exhaustion and aging, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
example 1
[0203] Extracts were prepared as follows:
[0204] 1. About 1 gram to about 5 grams (in various embodiments, about 1 gram, about 2 grams, about 3 grams, about 4 grams or about 5 grams) of dried Osmanthus plant parts and about 95 grams to about 99 grams of deionized water (the amount depends on the amount of dry Osmanthus such that total = 100 grams) combined.
[0205] 2. Heat this combination at about 40°C to about 80°C for about 2 hours to about 6 hours. The material was then cooled and filtered to remove suspended particulates.
[0206] 3. Apply the remaining solution (referred to herein as "composition A") to the protein of interest in the presence of glycated sugar and mix it with figure 1 Comparison of the following controls shown in : no treatment (ie, target protein plus sugar, without any other reagent present), 10 mM pyridoxamine (ie, target protein plus sugar plus 10 mM pyridoxamine). Such as figure 1 As can be seen in , when the AGE content of the differently ...
example 2
[0209] Extracts were prepared as follows:
[0210] 1. Combine dried Osmanthus plant parts in an amount of about 1 gram to about 5 grams and about 95 grams to about 99 grams of deionized water (total amount = 100 grams).
[0211] 2. Heat this combination at about 40°C to about 80°C for about 2 hours to about 6 hours. The material was then cooled and filtered to remove suspended particulates.
[0212] 3. Apply the remaining solution (referred to herein as "composition A") to the protein of interest in the presence of 10 mmoles of glyoxal (a reactive carbonyl compound) and mix it with figure 2 Comparison of the following controls shown in : no treatment (ie target protein plus glyoxal, without any other reagent present), 10 mM pyridoxamine (ie target protein plus glyoxal plus 10 mM pyridoxamine). Such as figure 1 with figure 2 As can be seen in , when the AGE content of the differently treated protein mixtures was determined by enzyme-linked immunoassay, the composition co...
example 3
[0214] A series of tests were performed to evaluate skin care compositions of the present technology to see if they could prevent BSA's target protein from being toxic to skin fibroblasts. This is done by first reacting BSA and a sugar (ribose) in buffered saline solution. Some of the reaction mixtures also contained pyridoxamine (the drug used here as a positive control known to prevent glycation) or a skin care composition of the present technology.
[0215]For this test, the sterile reaction mixture was incubated for 6.5 days at a temperature of about 45°C. After that, the reaction mixture was extensively dialyzed against deionized water, removing all small molecules (ribose, salts, etc.) from the mixture, leaving only BSA. The resulting BSA solution was then lyophilized (freeze-dried) to remove water, and the residue of BSA was redissolved in buffered saline solution. After measuring the respective protein concentrations of these solutions, each was added to replicate we...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More