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Dendrobium officinale kimura et migo endophytic fungi strain NT66G01 and application thereof

A technology of NT66G01 and Dendrobium candidum, applied in the field of microorganisms, can solve the problems of large market demand, no available wild resources, resource depletion, etc., and achieve the effects of increasing yield, promoting growth, and significant application value

Inactive Publication Date: 2015-05-20
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Dendrobium officinale Kimura et Migo is an epiphytic herb of the family Orchidaceae. It is a traditional Chinese medicine and is widely used in the treatment of chronic pharyngitis, obliterative peripheral vascular disease, cataract, glaucoma and other diseases, as well as various health products. The market demand is very large, long-term unrestrained excavation has caused its resources to be exhausted, and there are no wild resources available

Method used

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  • Dendrobium officinale kimura et migo endophytic fungi strain NT66G01 and application thereof
  • Dendrobium officinale kimura et migo endophytic fungi strain NT66G01 and application thereof
  • Dendrobium officinale kimura et migo endophytic fungi strain NT66G01 and application thereof

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Embodiment 1

[0022] The endophytic fungal strain NT66G01 of Dendrobium officinale according to the present invention was preserved in the China Center for Type Culture Collection (Wuhan University, Luojia Mountain, Wuchang, Wuhan City, Hubei Province, zip code 430072) on July 9, 2014, and the preservation number is CCTCC NO.M2014334 ; The genome 18s rDNA base sequence of this strain is shown in SEQ ID No.1.

[0023] The solid culture characteristic of described Dendrobium officinale endophytic fungal strain NT66G01 is:

[0024] When cultured on potato dextrose agar (PDA) medium at 28±1℃, the aerial hyphae were colorless at first and gradually turned white; the colonies were white fluffy, the mycelia were more developed, and the place near the medium was lavender for a long time. The white on the back slowly turns reddish-brown.

[0025] The liquid culture characteristic of described Dendrobium officinale endophytic fungal strain NT66G01 is:

[0026] a) Medium PDA, cultured in shake flask...

Embodiment 2

[0032] 1) get Dendrobium officinale endophytic fungal strain NT66G01 strain of the present invention, under aseptic conditions, pick a small amount of mycelium with an inoculation needle, insert a sterilized solid PDA medium test tube, and activate the culture at 28 ± 1 °C 72 hours;

[0033] 2) get the bacterial classification after the activation culture, under aseptic conditions, transfer into the sterilized liquid PDA seed medium, cultivate at 28 ± 1 ° C, 140 rpm shaker for 72 hours to obtain seed liquid;

[0034] 3) Under sterile conditions, insert into 500mL liquid PDA medium according to the inoculation amount of 10V% (volume percentage), and cultivate at 28±1°C, 140rpm shaker for 7 days;

[0035] 4) vacuum-filtering the fermentation broth, combining the grinding liquid of mycelium and liquid nitrogen with the filtrate and sterilizing at 121° C. for 20 minutes to obtain fungal elicitors;

[0036] 5) insert the fungal elicitor into the MS medium in an amount of 10% by vo...

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Abstract

The invention discloses a dendrobium officinale kimura et migo endophytic fungi strain NT66G01 and application thereof. The strain NT66G01 belongs to claviceps in taxonomy, is named Claviceps sp., and is preserved in China Center for Type Culture Collection on July 9, 2014, and the preservation number is CCTCC NO.M2014334. Genome 18s rDNA base sequence of the strain is shown in SEQ ID No.1. Experiment shows that fungus elicitor culture medium and biological bacterial fertilizer prepared by the dendrobium officinale kimura et migo endophytic fungi strain NT66G01 has obvious promotion function to growth of dendrobium officinale kimura et migo, and the dendrobium officinale kimura et migo endophytic fungi strain NT66G01can be used for artificial cultivation of the dendrobium officinale kimura et migo to improve yield of the dendrobium officinale kimura et migo and has obvious application value.

Description

technical field [0001] The invention relates to an endophytic fungal strain of Dendrobium officinale and its application, belonging to the technical field of microorganisms. Background technique [0002] Plant endophytic fungi refer to those fungi that live in various tissues and organs of healthy plants at a certain stage or all stages of their life history. Unlike mycorrhizal fungi, which only exist in the root system of plants, endophytic fungi live in the underground and Any tissue organ in the aerial part can exist (Faeth & Fagan, 2002). The study found that: endophytic fungi not only include mutually beneficial and neutral endosymbiotic fungi, but also those pathogenic bacteria lurking in the host; some endophytic fungi can promote the growth of host plants and enhance the host's resistance to stress. It can play a positive role in improving plant yield and quality by promoting the synthesis and accumulation of active ingredients in host plants. Therefore, the resear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05F11/08C12R1/645
CPCC05F5/002C12N1/145C12R2001/645C05F11/08
Inventor 黎万奎王莉莉马昭唐承晨张纯胡之璧王峥涛
Owner SHANGHAI UNIV OF T C M
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