Method for improving discriminating efficiency of thin layer chromatography
A thin-layer chromatography and thin-layer plate technology, which is applied in the field of biomedical preparations, can solve problems such as the influence of test results, thin-layer plate corrosion, and identification error judgments, and achieve the effects of accurate and reliable identification results, easy operation, and sensitive testing
Inactive Publication Date: 2015-05-20
KAMP PHARMA
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Problems solved by technology
[0002] Thin-layer chromatography is an important method in drug identification. Many identification processes require the use of chromogenic agents for color development, and the thin-layer plate needs to be heated. If the color development is not obvious, it is usually necessary to increase the heating temperature and heating time. In this case, if the color developer is corrosive, it is easy to cause corrosion to the thin-layer board, which will affect the color rendering effect
[0003] According to the requirements of "Chinese Pharmacopoeia", the thin-layer board is a self-made 1% sodium hydroxide solution silica gel G thin-layer board, so the thin-layer board is alkaline, and the color developer is 10% sulfuric acid ethanol solution, which is acidic. Therefore, after the color developer is sprayed, the color developer will undergo a neutralization reaction before the silica gel plate, so the color development effect of the color developer is reduced, and increasing the heating time and heating temperature will cause the silica gel plate to be absorbed by the sulfuric acid in the color developer. Carbonization and blackening also affect the color rendering effect, thereby affecting the final detection result, and even making wrong judgments on identification
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0015] Take the test solution and the reference solution and drop them on the same silica gel G thin-layer plate prepared with 1% sodium hydroxide solution. After being developed by the developing agent, air dry and spray with 10% sulfuric acid ethanol solution, and heat at 105°C until half point. The color is clear, and the heating time is more than 10 minutes.
Embodiment 2
[0017] Take the test solution and the reference solution and drop them on the same silica gel G thin-layer plate prepared by 1% sodium hydroxide solution. After being developed by the developing agent, spray it with 10% sulfuric acid ethanol solution, dry it, and blow it with a hair dryer. The dried TLC plate is heated for 2-3 minutes until the color develops.
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The invention discloses a method for improving the discriminating efficiency of thin layer chromatography. The method is characterized in that a thin layer plate aired by using an electric hair dryer is used to reach a chromogenic effect. The method replaces a 105DEG C heating step in a thin layer chromatography discriminating process to avoid the failure of the whole experiment due to forgetting and burning in order to improve the discriminating efficiency of the thin layer chromatography. The method has the advantages of simple operation, substantial discriminating effect and wide application prospect.
Description
technical field [0001] The invention belongs to the technical field of biomedical preparations, and in particular relates to a method for improving identification. Background technique [0002] Thin-layer chromatography is an important method in drug identification. Many identification processes require the use of chromogenic agents for color development, and the thin-layer plate needs to be heated. If the color development is not obvious, it is usually necessary to increase the heating temperature and heating time. In this case, if the color developer is corrosive, it is easy to cause corrosion to the thin-layer board, which will affect the color rendering effect. [0003] According to the requirements of "Chinese Pharmacopoeia", the thin-layer board is a self-made 1% sodium hydroxide solution silica gel G thin-layer board, so the thin-layer board is alkaline, and the color developer is 10% sulfuric acid ethanol solution, which is acidic. Therefore, after the color develop...
Claims
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IPC IPC(8): G01N30/90
Inventor 曾培安张凌霞李明吴健民贺莲张静刘娟
Owner KAMP PHARMA
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