Method for improving discriminating efficiency of thin layer chromatography

Abstract

The invention discloses a method for improving the discriminating efficiency of thin layer chromatography. The method is characterized in that a thin layer plate aired by using an electric hair dryer is used to reach a chromogenic effect. The method replaces a 105DEG C heating step in a thin layer chromatography discriminating process to avoid the failure of the whole experiment due to forgetting and burning in order to improve the discriminating efficiency of the thin layer chromatography. The method has the advantages of simple operation, substantial discriminating effect and wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of biomedical preparations, and in particular relates to a method for improving identification. Background technique [0002] Thin-layer chromatography is an important method in drug identification. Many identification processes require the use of chromogenic agents for color development, and the thin-layer plate needs to be heated. If the color development is not obvious, it is usually necessary to increase the heating temperature and heating time. In this case, if the color developer is corrosive, it is easy to cause corrosion to the thin-layer board, which will affect the color rendering effect. [0003] According to the requirements of "Chinese Pharmacopoeia", the thin-layer board is a self-made 1% sodium hydroxide solution silica gel G thin-layer board, so the thin-layer board is alkaline, and the color developer is 10% sulfuric acid ethanol solution, which is acidic. Therefore, after the color develop...

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Problems solved by technology

[0002] Thin-layer chromatography is an important method in drug identification. Many identification processes require the use of chromogenic agents for color development, and the thin-layer plate needs to be heated. If the color development is not obvious, it is usually necessary to increase the heating temperature and heating time. In this case, if the color developer is corrosive, it is easy to cause corrosion to the thin-layer board, which will affect the color rendering effect

[0003] According to the requirements of "Chinese Pharmacopoeia", the thin-layer board is a self-made 1% sodium hydroxide solution silica gel G thin-layer board, so the thin-layer board is alkaline, and the color developer is 10% sulfuric acid ethanol solution, which is acidic. Therefore, after the color developer is sprayed, the color developer will undergo a neutralization reaction before the silica gel plate, so the color development effect of the color developer is reduced, and increasing the heating time and heating temperature will cause the silica gel plate to be absorbed by the sulfuric acid in the color developer. Carbonization and blackening also affect the color rendering effect, thereby affecting the final detection result, and even making wrong judgments on identification