Application of compound XL-001 in preparation of medicines for treating chronic kidney disease
A technology for chronic kidney disease and compounds, applied in the field of preparation of drugs for the treatment of chronic kidney disease, to achieve the effect of delaying and inhibiting progress
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Embodiment 1
[0020] Example 1: Inhibition of XL-001 on renal fibrosis in UUO mice
[0021] 1. Experimental animals: BALB / c mice, male, weighing 20-22g, SPF grade. The animals were first weighed and numbered, and 18 healthy mice weighing 20-22 g were selected and randomly divided into 3 groups, 6 in each group. Including sham operation group, model control group and medication group.
[0022] XL-001 [N-(4-diethylamino-benzyl)-4-methoxy-N-p-tolyl-benzenesulfonamide; molecular formula C 25 h 30 N 2 o 3 S; molecular weight is 438.58] is a small molecular compound synthesized by the inventor's laboratory. The molecular structure of XL-001 is shown in the following formula:
[0023]
[0024] N-(4-Diethylamino-benzyl)-4-methoxy-N-p-tolyl-benzosulfonamide
[0025] 2. Treatment of each group
[0026] 1) Sham-operated group: After mice were anesthetized with 3% pentobarbital sodium at room temperature and 1ml / kg body weight, an incision was selected 1-2 cm below the left dorsal costal mar...
Embodiment 2
[0040] Example 2: Inhibition of XL-001 on renal fibrosis in UIRI mice
[0041] 1. Experimental animals: BABL / c mice, male, weighing 20-22g, SPF grade. The animals were first weighed and numbered, and 18 healthy mice weighing 20-22 g were selected and randomly divided into 3 groups, 6 in each group. Including sham operation group, model control group and medication group.
[0042] XL-001 (molecular formula C 25 h 30 N 2 o 3 S; molecular weight is 438.58) was synthesized by our laboratory.
[0043] 2. Treatment of each group
[0044] 1) Model control group: After mice were anesthetized with 3% pentobarbital sodium at room temperature and 1ml / kg body weight, the incision was made 1-2 cm below the left ventral costal margin; the skin, subcutaneous layer, muscle layer and peritoneum were cut layer by layer After the left kidney was found, the left renal artery was clamped with a vascular-arterial clip. During this period, the mouse was placed on a 37°C constant temperature inc...
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