A method and application of detecting distilled drunkenness
A technology of distilled wine and pure alcohol, which is applied in the field of detection of distilled intoxication, can solve the problems of lack of scientific and effective detection of distilled intoxication, and achieve the effect of improving health quality
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[0067] A method for detecting distilled intoxication, comprising the steps of:
[0068] 1. Preparation of detection reagents:
[0069] Use chromatographically pure ethanol and ultrapure water to prepare ethanol standard solutions with a concentration of 18 to 70% by volume, which are used to select the appropriate concentration when gavage mice with different doses; choose 2 or more than 2 kinds of Different varieties of liquor were tested; each liquor was selected with six gavage doses, converted according to the dose of pure alcohol: 0.356g / 100g, 0.435g / 100g, 0.514g / 100g, 0.593g / 100g, 0.672g / 100g, 0.751 g / 100g.
[0070] Wherein, the standard dose of ethanol solution is set, that is, the gavage dose of the ethanol standard solution to mice is converted according to the pure alcohol dose: 0.593g pure alcohol / 100g mice, and the measured value obtained after gavage to mice is a reference parameter.
[0071] 2. Selection of detection reaction objects: select 6 SPF-grade KM mice...
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