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Inhibitor aiming at miRNA-asu-miR-36f for specific expression of third-stage larvae of ascaris lumbricoides and application of inhibitor

A technology of inhibitors and roundworms, applied in the field of biological preparations, can solve problems such as drug residues, and achieve the effect of avoiding the problem of parasite drug resistance

Active Publication Date: 2015-06-24
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a miRNA—asu-miR-36f specifically expressed in third-stage larvae of roundworms against the problem of parasite drug resistance caused by existing antihelminth drugs, as well as the problem of drug residues in animal products and the environment. Inhibitor

Method used

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  • Inhibitor aiming at miRNA-asu-miR-36f for specific expression of third-stage larvae of ascaris lumbricoides and application of inhibitor
  • Inhibitor aiming at miRNA-asu-miR-36f for specific expression of third-stage larvae of ascaris lumbricoides and application of inhibitor
  • Inhibitor aiming at miRNA-asu-miR-36f for specific expression of third-stage larvae of ascaris lumbricoides and application of inhibitor

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Embodiment 1

[0021] 1. Preparation of Tertiary Larvae of Ascaris suum

[0022] Collect Ascaris suum adult females from the small intestine of naturally infected slaughtered pigs, dissect and remove about 1 / 2 of the fork end of the uterus, take out the eggs, put them into a mortar, and wash them with sterilized PBS solution (Na 2 HPO 4 12H 2 O 9.670g; KH 2 PO 4 1.429g; NaCl 2.125g; H 2 O 500ml) was slightly ground in a mortar, washed with PBS solution and 1.5% formalin solution, centrifuged twice, discarded the supernatant, put the precipitate into a petri dish, and cultivated in a 28°C incubator for 28 days. Shelling with 7.5% hypochlorous acid and proper shaking to obtain third-stage larvae of Ascaris suum.

[0023] 2. Preparation of culture medium

[0024] Prepare DMEM medium containing 50% fetal bovine serum, and add penicillin (1000U / ml), streptomycin (1mg / ml) and amphotericin (10μg / ml) to it, penicillin, streptomycin and amphotericin The addition of can prevent the growth of ...

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Abstract

The invention provides an inhibitor aiming at miRNA-asu-miR-36f for specific expression of third-stage larvae of ascaris lumbricoides. The inhibitor is capable of specifically inhibiting asu-miR-36f for specific expression of the third-stage larvae of ascaris lumbricoides. A nucleotide sequence of the inhibitor is UGACAAGGAUUGCAGCCCGGUGA. According to the inhibitor, through combination with the asu-miR-36f, the abundance of the asu-miR-36f is significantly reduced; development of the third-stage larvae of ascaris lumbricoides is significantly affected; and the infection rate is reduced. The miRNA-asu-miR-36f inhibitor for specific expression of the third-stage larvae of ascaris lumbricoides provided by the invention can be applied to affecting growth and development of the third-stage larvae of ascaris suum and ascaris lumbricoides, or functions related to infectivity.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, and in particular relates to an inhibitor of miRNA—asu-miR-36f specifically expressed in third-stage roundworm larvae and its application. Background technique [0002] Ascaris suum (Ascaris suum) is one of the common parasitic nematodes in pigs, which belongs to Ascaridae and lives in the small intestine of pigs. Ascariasis suum is prevalent widely, which greatly endangers the development of pig industry, especially in pig farms with poor sanitation, the infection rate is very high. The main manifestations of sick pigs are poor growth and development, and severe cases can cause growth and development stagnation, or even death. The long-term overuse of anti-helminth drugs has caused the problem of drug resistance in parasites distributed globally and the problem of drug residues in animal products and the environment. Therefore, it is imminent to speed up the research on Ascaris...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K48/00A61K31/7088A61P33/10
Inventor 徐民俊冯胜勇邵长春朱兴全
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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