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Transparent liquid for rapidly identifying oocyte nuclei of shrimps and crabs

An oocyte and transparent liquid technology is applied in the field of aquaculture to achieve the effects of shortening the artificial breeding time of shrimps and crabs, convenient use and convenient observation

Inactive Publication Date: 2015-08-05
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the yolk accumulated in the oocyte contains a variety of different substances such as proteins, lipids, and sugars, and the composition of the yolk of different species is very different, it can effectively transparent the transparent liquid of fish oocytes. but not transparent shrimp and crab oocytes

Method used

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  • Transparent liquid for rapidly identifying oocyte nuclei of shrimps and crabs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Transparent liquid treatment of Chinese mitten crab oocytes

[0018] I. Transparent liquid treatment method:

[0019] 1. Preparation of transparent liquid: Take absolute ethanol, glacial acetic acid and formaldehyde to prepare transparent liquid according to the volume ratio of 60:1:30, and prepare and use immediately.

[0020] 2. Oocyte transparency: dissect the mitten crab, take 100 mg of ovarian tissue and put it in a 15 ml centrifuge tube, add about 3 ml of transparent liquid, shake rapidly and vigorously (100-500 rpm), and make the oocyte In the free state, the color of mitten crab oocytes will change from black or purple to orange.

[0021] 3. Let stand for 1 to 2 minutes (or longer, no more than 60 minutes).

[0022] 4. Transfer the oocytes and transparent liquid to a petri dish or glass slide, and observe under a dissecting microscope with a black background. The result is as figure 1 B, A small white opaque spot was found at one end of the oocyte....

Embodiment 2

[0035] Example 2 Oocytes of Macrobrachium rosenbergii treated with transparent liquid

[0036] With the accumulation of yolk, the ovary of Macrobrachium rosenbergii gradually develops and becomes larger, and finally the entire carapace is almost filled with orange-yellow ovaries. Observed under the dissecting microscope, the free oocytes are round, oval and other shapes, and the cells are full of yolk ( figure 1 D). The methods of transparent liquid processing and HE detection are the same as in Example 1.

[0037] The formula of the transparent liquid is the same as in Example 1. Get 100 mg of ovarian tissue and place it in a centrifuge tube of 15 milliliters, add about 3 milliliters of the transparent liquid, and shake it violently (100-500 rpm) rapidly to make the oocytes in a free state; and Let it stand for 1 to 2 minutes (or longer, no more than 60 minutes). After treatment, the egg cells of Macrobrachium rosenbergii will become lighter in color.

[0038] Macrobrachi...

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Abstract

The invention relates to the field of aquaculture, and provides a transparent liquid which can be used for rapidly transparentizing oocytes of shrimps and crabs and a method for treating and detecting maturity conditions of the oocytes of the shrimps and crabs by using the transparent liquid. The transparent liquid is a mixture which is prepared from anhydrous ethyl alcohol, glacial acetic acid and formaldehyde at a volume ratio of 60:1:30. The transparent liquid provided by the invention can be used for treating the oocytes of the shrimps and crabs, can be used for effectively transparentizing the oocytes of the shrimps and crabs, and can be used for rapidly identifying germinal vesicles of the oocytes of the shrimps and crabs.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to a method for treating shrimp and crab egg cells, and a transparent liquid for quickly observing the growth and foaming of shrimp and crab egg cells. Background technique [0002] Shrimp and crab crustaceans are important aquaculture species, and the occurrence of oocytes mainly includes three periods: proliferation period, growth period and maturation period. Accurately judging the developmental stage of oocytes is of great practical value in timely grasping the time for artificially induced oocyte maturation in production, which can shorten the cycle of artificially assisted breeding of shrimp and crabs, and is also a good way to study the final maturation of shrimp and crab oocytes. An essential step of the mechanism. [0003] Similar to other yolk-egg species, shrimp and crab oocytes synthesize and reserve a large amount of yolk during the growth period for embryo development after...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
Inventor 丘高峰刘志强
Owner SHANGHAI OCEAN UNIV
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